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Literature summary for 1.11.1.14 extracted from

  • Ahmad, M.; Roberts, J.N.; Hardiman, E.M.; Singh, R.; Eltis, L.D.; Bugg, T.D.
    Identification of DypB from Rhodococcus jostii RHA1 as a lignin peroxidase (2011), Biochemistry, 50, 5096-5107.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodococcus jostii

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 1 mM, 5.4fold activation in assay with lignin. Presence of Mn2+ is required, Km value is 8.4 mM, and data are not consistent with DypB acting as a Mn peroxidase. Breakdown of wheat straw lignocellulose by recombinant enzyme is observed over 24-48 h in the presence of 1 mM MnCl2 Rhodococcus jostii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rhodococcus jostii enzyme DypB has a significant role in lignin degradation in Rhodococcus jostii RHA1, is able to oxidize both polymeric lignin and a lignin model compound, and appears to have both Mn(II) and lignin oxidation sites ?
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?

Organism

Organism UniProt Comment Textmining
Rhodococcus jostii Q0SE24
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol + H2O2
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Rhodococcus jostii vanillin + hydroxyacetaldehyde + guaiacol Calpha-Cbeta bond cleavage of substrate takes place. This reaction is inhibited by addition of diaphorase, consistent with a radical mechanism for C-C bond cleavage ?
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
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Rhodococcus jostii ? + H2O
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?
lignin + H2O2 substrate is Kraft lignin Rhodococcus jostii ? + H2O
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?
lignocellulose + H2O2 substrate is wheat straw lignocellulose Rhodococcus jostii ? + H2O
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?
additional information enzyme DypB has a significant role in lignin degradation in Rhodococcus jostii RHA1, is able to oxidize both polymeric lignin and a lignin model compound, and appears to have both Mn(II) and lignin oxidation sites Rhodococcus jostii ?
-
?

Synonyms

Synonyms Comment Organism
DypB
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Rhodococcus jostii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6
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substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) Rhodococcus jostii

General Information

General Information Comment Organism
physiological function wild-type Rhodococcus jostii RHA1 shows hydrogen peroxide-dependent activity, whereas the DypB knockout strain shows no observable activity in the presence or absence of hydrogen peroxide and a significant decrease in activity compared to that of the wild-type strain Rhodococcus jostii