Application | Comment | Organism |
---|---|---|
synthesis | immobilization of enzyme by entrapping in xerogel matrix of trimethoxysilane and proplytetramethoxysilane to maximum immobilization efficiency of 88.6%. The free and immobilized enzymes have optimum pH values of 6 and 5 while optimum temperatures are 60°C and 80°C, respectively. Immobilization enhances the activity and thermo-stability potential significantly and immobilized enzyme remains stable over broad pH and temperature range | Trametes versicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, about 80% inhibition of native enzyme, about 15% inhibition of enzyme immobilized in xerogel matrix of trimethoxysilane and proplytetramethoxysilane | Trametes versicolor | |
EDTA | 1 mM, about 75% inhibition of native enzyme, about 20% inhibition of enzyme immobilized in xerogel matrix of trimethoxysilane and proplytetramethoxysilane | Trametes versicolor | |
L-cysteine | 1 mM, complete inhibition of native enzyme, about 30% inhibition of enzyme immobilized in xerogel matrix of trimethoxysilane and proplytetramethoxysilane | Trametes versicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
veratryl alcohol | native enzyme, 25°C, pH 6.0 | Trametes versicolor | |
0.588 | - |
veratryl alcohol | enzyme immobilized in xerogel matrix of trimethoxysilane and proplytetramethoxysilane, 25°C, pH 5.0 | Trametes versicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, slight stimulation | Trametes versicolor | |
Fe2+ | 1 mM, slight stimulation | Trametes versicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
PAGE | Trametes versicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes versicolor | - |
- |
- |
Trametes versicolor IBL-04 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Trametes versicolor |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
553 | - |
pH 6.0, 25°C | Trametes versicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
veratryl alcohol + H2O2 | - |
Trametes versicolor | 3,4-dimethoxybenzaldehyde + H2O | - |
? | |
veratryl alcohol + H2O2 | - |
Trametes versicolor IBL-04 | 3,4-dimethoxybenzaldehyde + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Trametes versicolor |
Synonyms | Comment | Organism |
---|---|---|
LIP | - |
Trametes versicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
native enzyme | Trametes versicolor |
80 | - |
enzyme immobilized in xerogel matrix of trimethoxysilane and proplytetramethoxysilane | Trametes versicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
enzyme immobilized in xerogel matrix of trimethoxysilane and proplytetramethoxysilane | Trametes versicolor |
6 | - |
native enzyme | Trametes versicolor |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | native enzyme, stable for 1 h, enzyme immobilized in xerogel matrix of trimethoxysilane and proplytetramethoxysilane, stable for 24 h | Trametes versicolor |