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Literature summary for 1.11.1.16 extracted from

  • Ruiz-Duenas, F.J.; Pogni, R.; Morales, M.; Giansanti, S.; Mate, M.J.; Romero, A.; Martinez, M.J.; Basosi, R.; Martinez, A.T.
    Protein radicals in fungal versatile peroxidase: catalytic tryptophan radical in both compound I and compound II and studies on W164Y, W164H, and W164S variants (2009), J. Biol. Chem., 284, 7986-7994.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.16

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli W3110 Pleurotus eryngii

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme W164Y, sitting-drop vapor diffusion method, resolution 1.94 A Pleurotus eryngii

Protein Variants

Protein Variants Comment Organism
R257A/A260F site-directed mutagenesis Pleurotus eryngii
W164H site-directed mutagenesis, substitution of Trp-164 by a histidine, serine, or tyrosine residues causes a complete loss of activity on veratryl alcohol and Reactive Black 5 Pleurotus eryngii
W164S site-directed mutagenesis, substitution of Trp-164 by a histidine, serine, or tyrosine residues causes a complete loss of activity on veratryl alcohol and Reactive Black 5 Pleurotus eryngii
W164Y site-directed mutagenesis, substitution of Trp-164 by a histidine, serine, or tyrosine residues causes a complete loss of activity on veratryl alcohol and Reactive Black 5 Pleurotus eryngii
W164Y/R257A/A260F site-directed mutagenesis, substitution of Trp-164 by a histidine, serine, or tyrosine residues causes a complete loss of activity on veratryl alcohol and Reactive Black 5 Pleurotus eryngii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
 Reactive Black 5 native recombinant enzyme Pleurotus eryngii
0.0049
-
 Reactive Black 5 mutant enzyme R257A/A260F Pleurotus eryngii
0.076
-
 Mn2+ mutant enzyme W164Y/R257A/A260F Pleurotus eryngii
0.078
-
 Mn2+ mutant enzyme W164Y Pleurotus eryngii
0.11
-
 Mn2+ mutant enzyme W164S Pleurotus eryngii
0.133
-
 Mn2+ mutant enzyme W164H Pleurotus eryngii
0.15
-
 Mn2+ mutant enzyme R257A/A260F Pleurotus eryngii
4.13
-
 veratryl alcohol native recombinant enzyme Pleurotus eryngii
13.5
-
 veratryl alcohol mutant enzyme R257A/A260F Pleurotus eryngii

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii O94753
-
-

Purification (Commentary)

Purification (Comment) Organism
resource-Q chromatography Pleurotus eryngii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Mn2+ + H2O2
-
 Pleurotus eryngii ?
-
 ?
Reactive Black 5 + H2O2
-
 Pleurotus eryngii ?
-
 ?
veratryl alcohol + H2O2 + H+
-
 Pleurotus eryngii veratraldehyde + H2O
-
 ?

Synonyms

Synonyms Comment Organism
versatile peroxidase
-
 Pleurotus eryngii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5
-
 Reactive Black 5 native recombinant enzyme Pleurotus eryngii
9.1
-
 Reactive Black 5 mutant enzyme R257A/A260F Pleurotus eryngii
9.5
-
 veratryl alcohol native recombinant enzyme Pleurotus eryngii
17.9
-
 veratryl alcohol mutant enzyme R257A/A260F Pleurotus eryngii
164
-
 Mn2+ mutant enzyme W164Y Pleurotus eryngii
207
-
 Mn2+ mutant enzyme R257A/A260F Pleurotus eryngii
207
-
 Mn2+ mutant enzyme W164S Pleurotus eryngii
216
-
 Mn2+ mutant enzyme W164Y/R257A/A260F Pleurotus eryngii
275
-
 Mn2+ native recombinant enzyme Pleurotus eryngii
328
-
 Mn2+ mutant enzyme W164H Pleurotus eryngii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3
-
 veratryl alcohol mutant enzyme R257A/A260F Pleurotus eryngii
2.3
-
 veratryl alcohol native recombinant enzyme Pleurotus eryngii
1380
-
 Mn2+ mutant enzyme R257A/A260F Pleurotus eryngii
1520
-
 Mn2+ native recombinant enzyme Pleurotus eryngii
1610
-
 Reactive Black 5 native recombinant enzyme Pleurotus eryngii
1900
-
 Reactive Black 5 mutant enzyme R257A/A260F Pleurotus eryngii
1900
-
 Mn2+ mutant enzyme W164S Pleurotus eryngii
2110
-
 Mn2+ mutant enzyme W164Y Pleurotus eryngii
2470
-
 Mn2+ mutant enzyme W164H Pleurotus eryngii
2850
-
 Mn2+ mutant enzyme W164Y/R257A/A260F Pleurotus eryngii