KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
1,1-dimethyl-4-chloro-3,5-cyclohexanedione | nonhaem-type bromoperoxidase BPO 3 | Streptomyces griseus | |
0.0079 | - |
1,1-dimethyl-4-chloro-3,5-cyclohexanedione | nonhaem-type bromoperoxidase BPO 1a | Streptomyces griseus | |
6.4 | - |
Br- | nonhaem-type bromoperoxidase BPO 3 | Streptomyces griseus | |
95 | - |
H2O2 | nonhaem-type bromoperoxidase BPO 3 | Streptomyces griseus | |
160 | - |
Br- | nonhaem-type bromoperoxidase BPO 1a | Streptomyces griseus | |
430 | - |
H2O2 | nonhaem-type bromoperoxidase BPO 1a | Streptomyces griseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
3 * 31000, SDS-PAGE, nonhaem-type bromoperoxidase BPO 3 | Streptomyces griseus |
43000 | - |
2 * 43000, SDS-PAGE, nonhaem-type bromoperoxidase BPO 1b | Streptomyces griseus |
70000 | - |
gel filtration, nonhaem-type bromoperoxidase BPO 1a | Streptomyces griseus |
90000 | - |
gel filtration, nonhaem-type bromoperoxidase BPO 1b | Streptomyces griseus |
90000 | - |
gel filtration, nonhaem-type bromoperoxidase BPO 3 | Streptomyces griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | - |
strain Tü 6 | - |
Streptomyces griseus Tu6 | - |
strain Tü 6 | - |
Purification (Comment) | Organism |
---|---|
nonhaem-type bromoperoxidase BPO1a | Streptomyces griseus |
nonhaem-type bromoperoxidase BPO1b | Streptomyces griseus |
nonhaem-type bromoperoxidase BPO3 | Streptomyces griseus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptomyces griseus |
1.1 | - |
nonhaem-type bromoperoxidase BPO3 | Streptomyces griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione | i.e. monochlorodimedone | Streptomyces griseus | ? | - |
? | |
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione | i.e. monochlorodimedone | Streptomyces griseus Tu6 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 3400, SDS-PAGE, nonhaem-type bromoperoxidase BPO 1a | Streptomyces griseus |
dimer | 2 * 43000, SDS-PAGE, nonhaem-type bromoperoxidase BPO 1b | Streptomyces griseus |
trimer | 3 * 31000, SDS-PAGE, nonhaem-type bromoperoxidase BPO 3 | Streptomyces griseus |
Synonyms | Comment | Organism |
---|---|---|
nonhaem-type bromoperoxidase BPO 1a | - |
Streptomyces griseus |
nonhaem-type bromoperoxidase BPO 1b | - |
Streptomyces griseus |
nonhaem-type bromoperoxidase BPO 3 | - |
Streptomyces griseus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
nonhaem-type bromoperoxidase BPO 1a | Streptomyces griseus |
60 | - |
nonhaem-type bromoperoxidase BPO3 | Streptomyces griseus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
50 min, complete inactivation, nonhaem-type bromoperoxidase BPO 1a | Streptomyces griseus |
60 | - |
50 min, no loss of activity, nonhaem-type bromoperoxidase BPO 1b | Streptomyces griseus |
60 | - |
50 min, no loss of activity, nonhaem-type bromoperoxidase BPO 3 | Streptomyces griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
nonhaem-type bromoperoxidase BPO 1a | Streptomyces griseus |
4.5 | - |
nonhaem-type bromoperoxidase BPO3, at low concentrations of H2O2 (1.6 mM) the pH-optimum shifts from pH 4.5 to pH 4.0 | Streptomyces griseus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces griseus | isoelectric focusing, pH-range 3.5-9.5, nonhaem-type bromoperoxidase BPO 3 | - |
3.6 |
Streptomyces griseus | isoelectric focusing, pH-range 3.5-9.5, nonhaem-type bromoperoxidase BPO 1a | - |
4.6 |
Streptomyces griseus | isoelectric focusing, pH-range 3.5-9.5, nonhaem-type bromoperoxidase BPO 1b | - |
4.7 |