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Literature summary for 1.11.1.19 extracted from
- Purification and characterization of two DyP isozymes from Thanatephorus cucumeris Dec 1 specifically expressed in an air-membrane surface bioreactor (2009), J. Biosci. Bioeng., 107, 113-115.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
isozyme Dyp3, SDS-PAGE | Thanatephorus cucumeris |
| 58000 | - |
isozyme Dyp1 and isozyme Dyp2, SDS-PAGE | Thanatephorus cucumeris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thanatephorus cucumeris | - |
strain Dec 1 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Thanatephorus cucumeris |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| butyl-Toyopearl column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration | Thanatephorus cucumeris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17.1 | - |
unpurified supernatant, at pH 3.5 and 30°C | Thanatephorus cucumeris |
| 261 | - |
15.3fold purified isozyme Dyp2, at pH 3.5 and 30°C | Thanatephorus cucumeris |
| 732 | - |
42.8fold purified isozyme Dyp3, at pH 3.5 and 30°C | Thanatephorus cucumeris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-amino-2-sulfonyl-4-aminomethyl-9,10-anthraquinone + H2O2 + H+ | isozyme Dyp1 exhibits 77% relative activity, isozyme DyP2 exhibits 85% relative activity, and isozyme Dyp3 exhibits 150% relative activity compared to Reactive Blue 5 | Thanatephorus cucumeris | ? | - |
? |  |
| 2,6-dimethoxyphenol + H2O2 + H+ | isozyme Dyp1 exhibits 10% relative activity, isozyme DyP2 exhibits 11% relative activity, and isozyme Dyp3 exhibits 6.5% relative activity compared to Reactive Blue 5 | Thanatephorus cucumeris | ? | - |
? | |
| guaiacol + H2O2 + H+ | isozyme Dyp1 exhibits 5.6% relative activity, isozyme DyP2 exhibits 6.6% relative activity, and isozyme Dyp3 exhibits 37% relative activity compared to Reactive Blue 5 | Thanatephorus cucumeris | ? | - |
? | |
| additional information | isozymes DyP1, Dyp2 and Dyp3 do not oxidize veratryl alcohol and Reactive Red 202 | Thanatephorus cucumeris | ? | - |
? | |
| Reactive Blue 19 + H2O2 + H+ | isozyme Dyp1 exhibits 94% relative activity, isozyme DyP2 exhibits 84% relative activity, and isozyme Dyp3 exhibits 160% relative activity compared to Reactive Blue 5 | Thanatephorus cucumeris | oxidized Reactive Blue 19 + H2O | - |
? | |
| Reactive Blue 21 + H2O2 + H+ | isozyme Dyp1 exhibits 7.5% relative activity, isozyme DyP2 exhibits 9.6% relative activity, and isozyme Dyp3 exhibits 38% relative activity compared to Reactive Blue 5 | Thanatephorus cucumeris | oxidized Reactive Blue 21 + H2O | - |
? | |
| Reactive Blue 5 + H2O2 + H+ | isozyme DyP1, Dyp2 and Dyp3 show 100% activity towards Reactive Blue 5 | Thanatephorus cucumeris | oxidized Reactive Blue 5 + H2O | - |
? | |
| Reactive Red 123 + H2O2 + H+ | isozyme Dyp1 exhibits 0.72% relative activity, isozyme DyP2 exhibits 1.4% relative activity, and isozyme Dyp3 exhibits 0.8% relative activity compared to Reactive Blue 5 | Thanatephorus cucumeris | oxidized Reactive Red 123 + H2O | - |
? | |
| Reactive Red 225 + H2O2 + H+ | isozyme Dyp1 exhibits 8.2% relative activity, isozyme DyP2 exhibits 14% relative activity, and isozyme Dyp3 exhibits 9.9% relative activity compared to Reactive Blue 5 | Thanatephorus cucumeris | oxidized Reactive Red 225 + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DyP1 | isozyme | Thanatephorus cucumeris |
| DyP2 | isozyme | Thanatephorus cucumeris |
| DyP3 | isozyme | Thanatephorus cucumeris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
isozymes Dyp1, Dyp2, and Dyp3 | Thanatephorus cucumeris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | the activity of isozyme DyP3 is less than that of isozymes DyP2 and DyP1 at 35°C and 40°C. Isozymes DyP1, DyP2 and DyP3 exhibit approximately 75% activity at 20°C and about 90% activity at 30°C. Isozyme DyP1 shows about 75% activity at 40°C, isozyme DyP2 shows about 85% activity at 40°C, and isozyme DyP3 shows about 50% activity at 40°C. Isozymes DyP1, DyP2 and DyP3 show about 10% activity at 50°C and are completely inactivated at 60°C | Thanatephorus cucumeris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.2 | - |
isozymes Dyp1 and Dyp2 | Thanatephorus cucumeris |
| 3.6 | - |
isozyme Dyp3 | Thanatephorus cucumeris |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 5 | isozymes DyP1, DyP2 and DyP3 are completely inactivated at pH 2.5. Isozymes DyP1, DyP2 and DyP3 exhibit approximately 20% activity at pH 3.0. Isozymes DyP1 and Dyp2 show almost 100% activity at pH 3.5, isozyme DyP3 shows about 95% activity at pH 3.5. Isozyme DyP1 shows less than 50% activity at pH 4.0, isozymes DyP2 and Dyp3 show about 50% activity at pH 4.0. Isozymes Dyp1, DyP2 and Dyp3 show about 30% activity at pH 4.5 and about 20% activity at pH 5.0 | Thanatephorus cucumeris |
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