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Literature summary for 1.11.1.19 extracted from

  • Sugawara, K.; Yoshida, T.; Hirashima, R.; Toriumi, R.; Akiyama, H.; Kakuta, Y.; Ishige, Y.; Sugano, Y.
    Characterization of class V DyP-type peroxidase SaDyP1 from Streptomyces avermitilis and evaluation of SaDyPs expression in mycelium (2021), Int. J. Mol. Sci., 22, 8683 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.19

Inhibitors

Inhibitors Comment Organism Structure
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
 Streptomyces avermitilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.049
-
 Acid Blue 324 at pH 4.5 and 25°C Streptomyces avermitilis
0.61
-
 2,6-dimethoxyphenol at pH 4.0 and 25°C Streptomyces avermitilis
1.2
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.0 and 25°C Streptomyces avermitilis

Organism

Organism UniProt Comment Textmining
Streptomyces avermitilis
-
-
-
Streptomyces avermitilis ATCC31267
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and Superdex 75pg gel filtration Streptomyces avermitilis

Source Tissue

Source Tissue Comment Organism Textmining
aerial mycelium
-
 Streptomyces avermitilis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
 Streptomyces avermitilis oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
 Streptomyces avermitilis ATCC31267 oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Streptomyces avermitilis oxidized 2,6-dimethoxyphenol + H2O
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Streptomyces avermitilis ATCC31267 oxidized 2,6-dimethoxyphenol + H2O
-
 ?
Acid Blue 324 + H2O2 + H+
-
 Streptomyces avermitilis oxidized Acid Blue 324 + H2O
-
 ?
Acid Blue 324 + H2O2 + H+
-
 Streptomyces avermitilis ATCC31267 oxidized Acid Blue 324 + H2O
-
 ?
additional information no activity with AQ-2, M303, Reactive Black 5, and Reactive Red 33 Streptomyces avermitilis ?
-
-
additional information no activity with AQ-2, M303, Reactive Black 5, and Reactive Red 33 Streptomyces avermitilis ATCC31267 ?
-
-

Subunits

Subunits Comment Organism
? x * 50000, His6-tagged enzyme, SDS-PAGE Streptomyces avermitilis
? x * 49400, His6-tagged enzyme, calculated from amino acid sequence Streptomyces avermitilis

Synonyms

Synonyms Comment Organism
dye-decolorizing peroxidase
-
 Streptomyces avermitilis
DyP1
-
 Streptomyces avermitilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 the enzyme retains almost all of its initial activity against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,6-dimethoxyphenol after incubation at 30°C or 40°C for 2 h. The peroxidase activity of the enzyme against ABTS significantly decreases after incubating at 50°C for 1 h and is almost completely lost following incubation at 60°C for 30 min. On the other hand, the activity of the enzyme against 2,6-dimethoxyphenol is maintained at 50°C Streptomyces avermitilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.23
-
 2,6-dimethoxyphenol at pH 4.0 and 25°C Streptomyces avermitilis
0.79
-
 Acid Blue 324 at pH 4.5 and 25°C Streptomyces avermitilis
2.8
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.0 and 25°C Streptomyces avermitilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 the enzyme shows the highest activity for 2,6-dimethoxyphenol at pH 4.0 Streptomyces avermitilis
4.5
-
 the enzyme shows the highest activity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) or Acid Blue 324 at pH 4.5 Streptomyces avermitilis

Cofactor

Cofactor Comment Organism Structure
heme
-
 Streptomyces avermitilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
13
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.0 and 25°C Streptomyces avermitilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.38
-
 2,6-dimethoxyphenol at pH 4.0 and 25°C Streptomyces avermitilis
2.4
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.0 and 25°C Streptomyces avermitilis
16
-
 Acid Blue 324 at pH 4.5 and 25°C Streptomyces avermitilis