Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaCl | peroxidase activity is maximal at NaCl concentrations above 1 M, although catalase activity requires 2 M NaCl for optimal function | Halobacterium salinarum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | - |
Halobacterium salinarum | |
KCN | catalase and peroxidase activities are effectively inhibited by KCN | Halobacterium salinarum | |
additional information | catalatic activity of the purified H catalase-peroxidase is not inhibited by incubation for 2.5 h with 20 mM 3 amino-1,2,4-triazole | Halobacterium salinarum | |
NaN3 | catalase and peroxidase activities are effectively inhibited by NaN3 | Halobacterium salinarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
H2O2 | peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C | Halobacterium salinarum | |
3.7 | - |
H2O2 | catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C | Halobacterium salinarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60300 | - |
4 * 60300, estimated from SDS-PAGE | Halobacterium salinarum |
240000 | - |
gel filtration | Halobacterium salinarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halobacterium salinarum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Macrosphere GPC 300 column chromatography, hydroxylapatite column chromatography, and Sephadex G-150 gel filtration | Halobacterium salinarum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.4 | - |
crude extract, catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C | Halobacterium salinarum |
3.9 | - |
crude extract, peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C | Halobacterium salinarum |
43.2 | - |
after 12.7fold purification, catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C | Halobacterium salinarum |
104.3 | - |
after 29.6fold purification, peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C | Halobacterium salinarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3'-diaminobenzidine + H2O2 | - |
Halobacterium salinarum | oxidized 3,3'-diaminobenzidine + H2O | - |
? | |
4-aminoantipyrine + H2O2 | - |
Halobacterium salinarum | oxidized 4-aminoantipyrine + H2O | - |
? | |
ascorbate + H2O2 | - |
Halobacterium salinarum | monodehydroascorbate + H2O | - |
? | |
H2O2 | - |
Halobacterium salinarum | O2 + H2O | - |
? | |
NADH + H2O2 | - |
Halobacterium salinarum | ? | - |
? | |
NADPH + H2O2 | - |
Halobacterium salinarum | ? | - |
? | |
o-dianisidine + H2O2 | - |
Halobacterium salinarum | oxidized o-dianisidine + H2O | - |
? | |
reduced cytochrome c + H2O2 | - |
Halobacterium salinarum | oxidized cytochrome c + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 60300, estimated from SDS-PAGE | Halobacterium salinarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
peroxidase activity decreases rapidly above its maximum at 40°C | Halobacterium salinarum |
50 | - |
catalase activity | Halobacterium salinarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 90 | at 90°C, the catalatic activity is 20% greater than at 25°C. Peroxidatic activity declines after incubation at temperatures higher than 40°C, although at 70°C nearly 50% of the activity at 25°C is retained | Halobacterium salinarum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | catalase activity | Halobacterium salinarum |
6.5 | 8 | peroxidase activity | Halobacterium salinarum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | heme content of 1.43 per tetramer | Halobacterium salinarum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
NaN3 | apparent value, peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C | Halobacterium salinarum | |
0.05 | - |
KCN | apparent value, catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C | Halobacterium salinarum | |
0.05 | - |
KCN | apparent value, peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C | Halobacterium salinarum | |
0.0675 | - |
NaN3 | apparent value, catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C | Halobacterium salinarum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Halobacterium salinarum | isoelectric focusing | - |
3.8 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.4 | - |
peroxidase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 7.5, at 25°C | Halobacterium salinarum | H2O2 | |
4.5 | - |
catalase activity, in 50 mM potassium phosphate containing 2 M NaCl, pH 6.5, at 25°C | Halobacterium salinarum | H2O2 |