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Literature summary for 1.11.1.21 extracted from

  • Hochman, A.; Shemesh, A.
    Purification and characterization of a catalase-peroxidase from the photosynthetic bacterium Rhodopseudomonas capsulata (1987), J. Biol. Chem., 262, 6871-6876.
    View publication on PubMed
Search for more literature for 1.11.1.21

General Stability

General Stability Organism
both the catalatic and the peroxidatic activities are similarly inactivated by exposure to photooxidative conditions. The enzyme is reduced by sodium dithionite Rhodobacter capsulatus

Inhibitors

Inhibitors Comment Organism Structure
azide
-
 Rhodobacter capsulatus
cyanide bo th enzymic activities of catalase-peroxidase are equally inhibited by cyanide Rhodobacter capsulatus
H2O2 both the catalatic and the peroxidatic activities are similarly inactivated by treatment with 1 mM H2O2 Rhodobacter capsulatus
hydroxylamine
-
 Rhodobacter capsulatus
additional information p-chloromercuribenzoate at concentrations up to 100 mM does not inhibit the enzyme Rhodobacter capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
236000
-
 gel filtration Rhodobacter capsulatus

Organic Solvent Stability

Organic Solvent Comment Organism
chloroform both the catalatic and the peroxidatic activities are similarly inactivated by exposure to chloroform Rhodobacter capsulatus
Ethanol both the catalatic and the peroxidatic activities are similarly inactivated by exposure to ethanol Rhodobacter capsulatus

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diaminobenzidine + H2O2
-
 Rhodobacter capsulatus oxidized diaminobenzidine + H2O
-
 ?
H2O2
-
 Rhodobacter capsulatus O2 + H2O
-
 ?
additional information no reaction is detected with guaiacol or with ascorbate Rhodobacter capsulatus ?
-
 ?
o-dianisidine + H2O2
-
 Rhodobacter capsulatus oxidized o-dianisidine + H2O
-
 ?
pyrogallol + H2O2
-
 Rhodobacter capsulatus ? + H2O
-
 ?

Subunits

Subunits Comment Organism
homotetramer
-
 Rhodobacter capsulatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 both the catalatic and the peroxidatic activities are similarly inactivated by heating to 50°C Rhodobacter capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.3 peroxidatic activity Rhodobacter capsulatus
6 6.5 catalatic activity Rhodobacter capsulatus

Cofactor

Cofactor Comment Organism Structure
heme there are 1-1.2 molecules of protoheme IX per tetrameric molecule Rhodobacter capsulatus

pI Value

Organism Comment pI Value Maximum pI Value
Rhodobacter capsulatus isoelectric focusing
-
 4.5

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00215
-
 catalatic activity, in 100 mM potassium phosphate, pH 6.3, at 30°C Rhodobacter capsulatus hydroxylamine
0.022
-
 catalatic activity, in 100 mM potassium phosphate, pH 6.3, at 30°C Rhodobacter capsulatus cyanide
0.023
-
 peroxidatic activity, in 100 mM potassium phosphate, pH 5.2, at 30°C Rhodobacter capsulatus cyanide
0.078
-
 peroxidatic activity, in 100 mM potassium phosphate, pH 5.2, at 30°C Rhodobacter capsulatus hydroxylamine
0.15
-
 catalatic activity, in 100 mM potassium phosphate, pH 6.3, at 30°C Rhodobacter capsulatus azide
0.73
-
 peroxidatic activity, in 100 mM potassium phosphate, pH 5.2, at 30°C Rhodobacter capsulatus azide