Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Escherichia coli |
expressed in Escherichia coli | Geobacillus stearothermophilus |
expressed in Escherichia coli | Rhodobacter capsulatus |
expressed in Escherichia coli | Mycobacterium tuberculosis |
expressed in Escherichia coli | Synechocystis sp. |
expressed in Escherichia coli | Archaeoglobus fulgidus |
expressed in Escherichia coli | Burkholderia pseudomallei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | highly sensitive | Archaeoglobus fulgidus | |
azide | highly sensitive | Burkholderia pseudomallei | |
azide | highly sensitive | Escherichia coli | |
azide | highly sensitive | Geobacillus stearothermophilus | |
azide | highly sensitive | Mycobacterium tuberculosis | |
azide | highly sensitive | Rhodobacter capsulatus | |
azide | highly sensitive | Synechocystis sp. | |
cyanide | highly sensitive | Archaeoglobus fulgidus | |
cyanide | highly sensitive | Burkholderia pseudomallei | |
cyanide | highly sensitive | Escherichia coli | |
cyanide | highly sensitive | Geobacillus stearothermophilus | |
cyanide | highly sensitive | Mycobacterium tuberculosis | |
cyanide | highly sensitive | Rhodobacter capsulatus | |
cyanide | highly sensitive | Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase reaction, at pH 4.3 and 25°C | Synechocystis sp. | |
0.016 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase reaction, at pH 4.5 and 25°C | Archaeoglobus fulgidus | |
0.016 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase reaction, at pH 5.0 and 25°C | Rhodobacter capsulatus | |
0.024 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase reaction, at pH 4.3 and 25°C | Escherichia coli | |
0.031 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase reaction, at pH 4.0 and 25°C | Geobacillus stearothermophilus | |
0.06 | - |
H2O2 | peroxidase reaction, at pH 4.3 and 25°C | Escherichia coli | |
0.067 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase reaction, at pH 4.8 and 25°C | Mycobacterium tuberculosis | |
0.095 | - |
H2O2 | peroxidase reaction, at pH 4.5 and 25°C | Archaeoglobus fulgidus | |
0.21 | - |
H2O2 | peroxidase reaction, at pH 4.0 and 25°C | Geobacillus stearothermophilus | |
0.3 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase reaction, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.36 | - |
H2O2 | peroxidase reaction, at pH 4.8 and 25°C | Mycobacterium tuberculosis | |
0.7 | - |
H2O2 | peroxidase reaction, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.83 | - |
H2O2 | peroxidase reaction, at pH 5.0 and 25°C | Rhodobacter capsulatus | |
1 | - |
H2O2 | peroxidase reaction, at pH 4.3 and 25°C | Synechocystis sp. | |
2.4 | - |
H2O2 | catalase reaction, at pH 7.0 and 37°C | Mycobacterium tuberculosis | |
3.1 | - |
H2O2 | catalase reaction, at pH 7.0 and 37°C | Synechocystis sp. | |
3.7 | - |
H2O2 | catalase reaction, at pH 7.0 and 37°C | Geobacillus stearothermophilus | |
3.7 | - |
H2O2 | catalase reaction, at pH 7.0 and 37°C | Rhodobacter capsulatus | |
3.8 | - |
H2O2 | catalase reaction, at pH 7.0 and 37°C | Archaeoglobus fulgidus | |
4.2 | - |
H2O2 | catalase reaction, at pH 7.0 and 37°C | Escherichia coli | |
4.5 | - |
H2O2 | catalase reaction, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
20 | - |
H2O2 | catalase reaction, at pH 5.5-6.0 and 37°C | Synechocystis sp. | |
30 | - |
H2O2 | catalase reaction, at pH 5.5-6.0 and 37°C | Rhodobacter capsulatus | |
32 | - |
H2O2 | catalase reaction, at pH 5.5-6.0 and 37°C | Archaeoglobus fulgidus | |
35 | - |
H2O2 | catalase reaction, at pH 5.5-6.0 and 37°C | Escherichia coli | |
56 | - |
H2O2 | catalase reaction, at pH 5.5-6.0 and 37°C | Burkholderia pseudomallei | |
90 | - |
H2O2 | catalase reaction, at pH 5.5-6.0 and 37°C | Geobacillus stearothermophilus | |
225 | - |
H2O2 | catalase reaction, at pH 5.5-6.0 and 37°C | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
about 80000, SDS-PAGE | Escherichia coli |
80000 | - |
about 80000, SDS-PAGE | Geobacillus stearothermophilus |
80000 | - |
about 80000, SDS-PAGE | Rhodobacter capsulatus |
80000 | - |
about 80000, SDS-PAGE | Mycobacterium tuberculosis |
80000 | - |
about 80000, SDS-PAGE | Synechocystis sp. |
80000 | - |
about 80000, SDS-PAGE | Archaeoglobus fulgidus |
80000 | - |
about 80000, SDS-PAGE | Burkholderia pseudomallei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
- |
- |
Burkholderia pseudomallei | - |
- |
- |
Escherichia coli | - |
- |
- |
Geobacillus stearothermophilus | - |
- |
- |
Mycobacterium tuberculosis | - |
- |
- |
Rhodobacter capsulatus | - |
- |
- |
Synechocystis sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and DEAE-cellulose column chromatography | Escherichia coli |
ammonium sulfate precipitation and DEAE-cellulose column chromatography | Geobacillus stearothermophilus |
ammonium sulfate precipitation and DEAE-cellulose column chromatography | Rhodobacter capsulatus |
ammonium sulfate precipitation and DEAE-cellulose column chromatography | Mycobacterium tuberculosis |
ammonium sulfate precipitation and DEAE-cellulose column chromatography | Synechocystis sp. |
ammonium sulfate precipitation and DEAE-cellulose column chromatography | Archaeoglobus fulgidus |
ammonium sulfate precipitation and DEAE-cellulose column chromatography | Burkholderia pseudomallei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
peroxidase specific activity, using o-dianisidine as substrate, at pH 5.0 and 25°C | Rhodobacter capsulatus |
2 | - |
peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 5.0 and 25°C | Rhodobacter capsulatus |
3.2 | - |
peroxidase specific activity, using o-dianisidine as substrate, at pH 4.5 and 25°C | Archaeoglobus fulgidus |
4.8 | - |
peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.5 and 25°C | Burkholderia pseudomallei |
5.3 | - |
peroxidase specific activity, using o-dianisidine as substrate, at pH 4.0 and 25°C | Geobacillus stearothermophilus |
5.3 | - |
peroxidase specific activity, using o-dianisidine as substrate, at pH 4.5 and 25°C | Burkholderia pseudomallei |
5.5 | - |
peroxidase specific activity, using o-dianisidine as substrate, at pH 4.3 and 25°C | Synechocystis sp. |
5.9 | - |
peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.0 and 25°C | Geobacillus stearothermophilus |
6.4 | - |
peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.3 and 25°C | Synechocystis sp. |
8.3 | - |
peroxidase specific activity, using o-dianisidine as substrate, at pH 4.3 and 25°C | Escherichia coli |
8.8 | - |
peroxidase specific activity, using o-dianisidine as substrate, at pH 4.8 and 25°C | Mycobacterium tuberculosis |
10 | - |
peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.8 and 25°C | Mycobacterium tuberculosis |
11 | - |
peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.3 and 25°C | Escherichia coli |
12 | - |
peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.5 and 25°C | Archaeoglobus fulgidus |
2200 | - |
catalase specific activity, at pH 7.0 and 37°C | Escherichia coli |
3120 | - |
catalase specific activity, at pH 7.0 and 37°C | Geobacillus stearothermophilus |
3630 | - |
catalase specific activity, at pH 7.0 and 37°C | Burkholderia pseudomallei |
4450 | - |
catalase specific activity, at pH 7.0 and 37°C | Mycobacterium tuberculosis |
4830 | - |
catalase specific activity, at pH 7.0 and 37°C | Rhodobacter capsulatus |
5280 | - |
catalase specific activity, at pH 7.0 and 37°C | Archaeoglobus fulgidus |
5420 | - |
catalase specific activity, at pH 7.0 and 37°C | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Escherichia coli | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Geobacillus stearothermophilus | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Rhodobacter capsulatus | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Mycobacterium tuberculosis | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Synechocystis sp. | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Archaeoglobus fulgidus | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Burkholderia pseudomallei | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
H2O2 | - |
Escherichia coli | O2 + H2O | - |
? | |
H2O2 | - |
Geobacillus stearothermophilus | O2 + H2O | - |
? | |
H2O2 | - |
Rhodobacter capsulatus | O2 + H2O | - |
? | |
H2O2 | - |
Mycobacterium tuberculosis | O2 + H2O | - |
? | |
H2O2 | - |
Synechocystis sp. | O2 + H2O | - |
? | |
H2O2 | - |
Archaeoglobus fulgidus | O2 + H2O | - |
? | |
H2O2 | - |
Burkholderia pseudomallei | O2 + H2O | - |
? | |
o-dianisidine + H2O2 | - |
Escherichia coli | oxidized o-dianisidine + H2O | - |
? | |
o-dianisidine + H2O2 | - |
Geobacillus stearothermophilus | oxidized o-dianisidine + H2O | - |
? | |
o-dianisidine + H2O2 | - |
Rhodobacter capsulatus | oxidized o-dianisidine + H2O | - |
? | |
o-dianisidine + H2O2 | - |
Mycobacterium tuberculosis | oxidized o-dianisidine + H2O | - |
? | |
o-dianisidine + H2O2 | - |
Synechocystis sp. | oxidized o-dianisidine + H2O | - |
? | |
o-dianisidine + H2O2 | - |
Archaeoglobus fulgidus | oxidized o-dianisidine + H2O | - |
? | |
o-dianisidine + H2O2 | - |
Burkholderia pseudomallei | oxidized o-dianisidine + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KatG | - |
Escherichia coli |
KatG | - |
Geobacillus stearothermophilus |
KatG | - |
Rhodobacter capsulatus |
KatG | - |
Mycobacterium tuberculosis |
KatG | - |
Synechocystis sp. |
KatG | - |
Archaeoglobus fulgidus |
KatG | - |
Burkholderia pseudomallei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
the enzyme is completely inactive after less than 1 min at 65°C | Rhodobacter capsulatus |
65 | - |
the enzyme is completely inactive after less than 1 min at 65°C | Synechocystis sp. |
65 | - |
the enzyme is completely inactive after less than 1 min at 65°C | Burkholderia pseudomallei |
65 | - |
the enzyme is completely inactive after less than 1.5 min at 65°C | Escherichia coli |
65 | - |
the enzyme is completely inactive after less than 1.5 min at 65°C | Mycobacterium tuberculosis |
65 | - |
the enzyme is completely inactive after more than 40 min at 65°C | Geobacillus stearothermophilus |
65 | - |
the enzyme is completely inactive after more than 40 min at 65°C | Archaeoglobus fulgidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.7 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase reaction, at pH 5.0 and 25°C | Rhodobacter capsulatus | |
7.9 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase reaction, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
11 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase reaction, at pH 4.0 and 25°C | Geobacillus stearothermophilus | |
13 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase reaction, at pH 4.3 and 25°C | Synechocystis sp. | |
17 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase reaction, at pH 4.5 and 25°C | Archaeoglobus fulgidus | |
19 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase reaction, at pH 4.8 and 25°C | Mycobacterium tuberculosis | |
25 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase reaction, at pH 4.3 and 25°C | Escherichia coli | |
2950 | - |
H2O2 | catalase reaction, at pH 7.0 and 37°C | Escherichia coli | |
4300 | - |
H2O2 | catalase reaction, at pH 7.0 and 37°C | Geobacillus stearothermophilus | |
4350 | - |
H2O2 | catalase reaction, at pH 7.0 and 37°C | Mycobacterium tuberculosis | |
4970 | - |
H2O2 | catalase reaction, at pH 5.5-6.0 and 37°C | Escherichia coli | |
5680 | - |
H2O2 | catalase reaction, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
6640 | - |
H2O2 | catalase reaction, at pH 7.0 and 37°C | Rhodobacter capsulatus | |
7600 | - |
H2O2 | catalase reaction, at pH 5.5-6.0 and 37°C | Geobacillus stearothermophilus | |
7630 | - |
H2O2 | catalase reaction, at pH 7.0 and 37°C | Synechocystis sp. | |
7770 | - |
H2O2 | catalase reaction, at pH 7.0 and 37°C | Archaeoglobus fulgidus | |
8000 | - |
H2O2 | catalase reaction, at pH 5.5-6.0 and 37°C | Synechocystis sp. | |
10200 | - |
H2O2 | catalase reaction, at pH 5.5-6.0 and 37°C | Mycobacterium tuberculosis | |
14100 | - |
H2O2 | catalase reaction, at pH 5.5-6.0 and 37°C | Rhodobacter capsulatus | |
15680 | - |
H2O2 | catalase reaction, at pH 5.5-6.0 and 37°C | Archaeoglobus fulgidus | |
15900 | - |
H2O2 | catalase reaction, at pH 5.5-6.0 and 37°C | Burkholderia pseudomallei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Geobacillus stearothermophilus |
4.3 | - |
- |
Escherichia coli |
4.3 | - |
- |
Synechocystis sp. |
4.5 | - |
- |
Archaeoglobus fulgidus |
4.5 | - |
- |
Burkholderia pseudomallei |
4.8 | - |
- |
Mycobacterium tuberculosis |
5 | - |
- |
Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Escherichia coli | |
heme | - |
Geobacillus stearothermophilus | |
heme | - |
Rhodobacter capsulatus | |
heme | - |
Mycobacterium tuberculosis | |
heme | - |
Synechocystis sp. | |
heme | - |
Archaeoglobus fulgidus | |
heme | - |
Burkholderia pseudomallei |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0002 | - |
at pH 7.0 and 37°C | Mycobacterium tuberculosis | azide | |
0.002 | - |
at pH 7.0 and 37°C | Geobacillus stearothermophilus | azide | |
0.007 | - |
at pH 7.0 and 37°C | Escherichia coli | azide | |
0.008 | - |
at pH 7.0 and 37°C | Rhodobacter capsulatus | azide | |
0.008 | - |
at pH 7.0 and 37°C | Burkholderia pseudomallei | azide | |
0.013 | - |
at pH 7.0 and 37°C | Synechocystis sp. | azide | |
0.02 | - |
at pH 7.0 and 37°C | Archaeoglobus fulgidus | azide | |
0.04 | - |
at pH 7.0 and 37°C | Burkholderia pseudomallei | cyanide | |
0.05 | - |
at pH 7.0 and 37°C | Escherichia coli | cyanide | |
0.05 | - |
at pH 7.0 and 37°C | Geobacillus stearothermophilus | cyanide | |
0.08 | - |
at pH 7.0 and 37°C | Rhodobacter capsulatus | cyanide | |
0.1 | - |
at pH 7.0 and 37°C | Mycobacterium tuberculosis | cyanide | |
0.15 | - |
at pH 7.0 and 37°C | Archaeoglobus fulgidus | cyanide | |
0.17 | - |
at pH 7.0 and 37°C | Synechocystis sp. | cyanide |