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Literature summary for 1.11.1.21 extracted from
- A catalase-peroxidase for oxidation of beta-lactams to their (R)-sulfoxides (2012), Biores. Technol., 115, 102-110.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | biocatalytic method for stereoselective oxidation of beta-lactams, such as penicillin-G, penicillin-V and cephalosporin-G to their (R)-sulfoxides | Bacillus pumilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | 0.05 mM, 80% inhibition of peroxidase activity, 88% inhibition of catalyse activity | Bacillus pumilus |  |
| KCN | 0.05 mM, 30% inhibition of peroxidase activity, 22% inhibition of catalyse activity | Bacillus pumilus | |
| Sodium azide | 25 mM, 30% inhibition of peroxidase activity, 100% inhibition of catalyse activity | Bacillus pumilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.82 | - |
H2O2 | catalase activity, pH 7.0, 37°C | Bacillus pumilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
gel filtration | Bacillus pumilus |
| 62140 | - |
1 * 62400, SDS-PAGE, 1 * 62140, MALDI-TOF | Bacillus pumilus |
| 62400 | - |
1 * 62400, SDS-PAGE, 1 * 62140, MALDI-TOF | Bacillus pumilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus pumilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus pumilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 743 | - |
peroxidase activity, pH 7.0, 37°C | Bacillus pumilus |
| 818 | - |
catalase activity, pH 7.0, 37°C | Bacillus pumilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 H2O2 | - |
Bacillus pumilus | O2 + 2 H2O | - |
? | |
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Bacillus pumilus | ? + H2O | - |
? | |
| penicillin G + H2O2 | - |
Bacillus pumilus | penicillin G (R)-sulfoxide + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 62400, SDS-PAGE, 1 * 62140, MALDI-TOF | Bacillus pumilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Bacillus pumilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
sharp decrease in activity above | Bacillus pumilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bacillus pumilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | more than 75% of maximum activity within | Bacillus pumilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Bacillus pumilus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1300 | - |
H2O2 | catalase activity, pH 7.0, 37°C | Bacillus pumilus | |
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