Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Streptomyces wedmorensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, complete inactivation | Streptomyces wedmorensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | mononuclear non-heme iron enzyme. Substrate binds near, and perhaps to, the active site Fe2+ and in doing so organizes the center so that effectively one species is present | Streptomyces wedmorensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
4 * 21000, SDS-PAGE | Streptomyces wedmorensis |
21210 | - |
4 * 21210, calculated from sequence | Streptomyces wedmorensis |
89000 | - |
gel filtration | Streptomyces wedmorensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-hydroxypropylphosphonic acid + 2 NADH + O2 | Streptomyces wedmorensis | - |
cis-(1R,2S)-epoxypropylphosphonic acid + 2 H2O + 2 NAD+ | i.e. fosfomycin | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces wedmorensis | Q56185 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces wedmorensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-hydroxypropylphosphonic acid + 2 NADH + O2 | - |
Streptomyces wedmorensis | cis-(1R,2S)-epoxypropylphosphonic acid + 2 H2O + 2 NAD+ | i.e. fosfomycin | ? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 21000, SDS-PAGE | Streptomyces wedmorensis |
tetramer | 4 * 21210, calculated from sequence | Streptomyces wedmorensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | FMN or FAD increase level of fosfomycin production. The effect of FMN is slightly better than that of FAD. The flavin coenzyme is not likely to be an integral part of the epoxidase itself, but it may serve as a surrogate for the putative electron mediator in the in vitro assay | Streptomyces wedmorensis | |
FMN | FMN or FAD increase level of fosfomycin production. The effect of FMN is slightly better than that of FAD. The flavin coenzyme is not likely to be an integral part of the epoxidase itself, but it may serve as a surrogate for the putative electron mediator in the in vitro assay | Streptomyces wedmorensis | |
NADH | NADH is a necessary component for (S)-2-hydroxypropylphosphonic acid epoxidation and the overall catalysis is a four-electron redox reaction | Streptomyces wedmorensis |