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Literature summary for 1.11.1.3 extracted from
- A dual function alpha-dioxygenase-peroxidase and NAD+ oxidoreductase active enzyme from germinating pea rationalizing alpha-oxidation of fatty acids in plants (2000), Plant Physiol., 123, 1545-1551.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.055 | - |
lauric acid | - |
Pisum sativum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
alpha,beta, 1 * 70000 + 1 * 50000, 70 kD subunit shows EC 1.11.1.3 activity, 50 kD subunit shows NAD+ oxidoreductase activity | Pisum sativum |
| 70000 | - |
alpha,beta, 1 * 70000 + 1 * 50000, 70 kD subunit shows EC 1.11.1.3 activity, 50 kD subunit shows NAD+ oxidoreductase activity | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| palmitate + H2O2 | Pisum sativum | - |
pentadecanal + CO2 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.9 | - |
purified enzyme with lauric acid as substrate | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lauric acid + H2O2 | - |
Pisum sativum | undecanal + CO2 + H2O | - |
? | |
| palmitate + H2O2 | - |
Pisum sativum | pentadecanal + CO2 + H2O | - |
? | |
| palmitate + H2O2 | - |
Pisum sativum | pentadecanal + CO2 + H2O | (R)-2-hydroxypalmitic acid and pentadecanoic acid also detected as products | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha,beta, 1 * 70000 + 1 * 50000, 70 kD subunit shows EC 1.11.1.3 activity, 50 kD subunit shows NAD+ oxidoreductase activity | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Pisum sativum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8 | highest activity | Pisum sativum |
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Release 2023.1 (January 2023)
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