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Literature summary for 1.11.1.5 extracted from

  • Pfister, T.D.; Mirarefi, A.Y.; Gengenbach, A.J.; Zhao, X.; Danstrom, C.; Conatser, N.; Gao, Y.G.; Robinson, H.; Zukoski, C.F.; Wang, A.H.; Lu, Y.
    Kinetic and crystallographic studies of a redesigned manganese-binding site in cytochrome c peroxidase (2007), J. Biol. Inorg. Chem., 12, 126-137.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
of mutant D37E/V45E/H181E in a metal-free form and with Co2+ at the designed Mn2+ site, mutant is a close structural model of the Mn2+ binding site in manganese peroxidase Phanerodontia chrysosporium

Protein Variants

Protein Variants Comment Organism
D37E/P44D/V45D redesign of a manganese-binding site, ratio kcat/KM values for manganese oxidation is 0.33 per mM and s at pH 5.0 Phanerodontia chrysosporium
D37E/V45E/H181E redesign of a manganese-binding site, ratio kcat/KM values for manganese oxidation is 0.25 per mM and s at pH 5.0 Phanerodontia chrysosporium
G41E/V45E/H181D redesign of a manganese-binding site, ratio kcat/KM values for manganese oxidation is 0.10 per mM and s at pH 5.0 Phanerodontia chrysosporium
G41E/V45E/W51F/H181D/W191F redesign of a manganese-binding site, ratio kcat/KM values for manganese oxidation is 0.6 per mM and s at pH 5.0 Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
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white rot fungus
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