Crystallization (Comment) | Organism |
---|---|
of mutant D37E/V45E/H181E in a metal-free form and with Co2+ at the designed Mn2+ site, mutant is a close structural model of the Mn2+ binding site in manganese peroxidase | Phanerodontia chrysosporium |
Protein Variants | Comment | Organism |
---|---|---|
D37E/P44D/V45D | redesign of a manganese-binding site, ratio kcat/KM values for manganese oxidation is 0.33 per mM and s at pH 5.0 | Phanerodontia chrysosporium |
D37E/V45E/H181E | redesign of a manganese-binding site, ratio kcat/KM values for manganese oxidation is 0.25 per mM and s at pH 5.0 | Phanerodontia chrysosporium |
G41E/V45E/H181D | redesign of a manganese-binding site, ratio kcat/KM values for manganese oxidation is 0.10 per mM and s at pH 5.0 | Phanerodontia chrysosporium |
G41E/V45E/W51F/H181D/W191F | redesign of a manganese-binding site, ratio kcat/KM values for manganese oxidation is 0.6 per mM and s at pH 5.0 | Phanerodontia chrysosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
white rot fungus | - |