Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | CcP requires reductive activation for full activity | Shewanella oneidensis |
Cloned (Comment) | Organism |
---|---|
expression of maltose-binding-protein-fusion and tag-free CcP in Escherichia coli strain JM 109, the presence of the MBP tag affects the availability of certain binding sites, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Shewanella oneidensis |
Protein Variants | Comment | Organism |
---|---|---|
M219Q/F247N | site-directed mutagenesis | Shewanella oneidensis |
P75T/H81K/E84Q | site-directed mutagenesis | Shewanella oneidensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Shewanella oneidensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella oneidensis | - |
gene SO2178 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Shewanella oneidensis |
Synonyms | Comment | Organism |
---|---|---|
CCP | - |
Shewanella oneidensis |
cytochrome c peroxidase | - |
Shewanella oneidensis |
diheme cytochrome c peroxidase | - |
Shewanella oneidensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Shewanella oneidensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Shewanella oneidensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | diheme cytochrome c peroxidase | Shewanella oneidensis |
General Information | Comment | Organism |
---|---|---|
additional information | CcP requires reductive activation for full activity. The rates of catalysis and activation differ between maltose-binding-protein-fusion and tag-free CcP and also depend on the identity of the electron donor | Shewanella oneidensis |