Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-aminoantipyrine | inhibition of catalase activity | Bacillus sp. (in: Bacteria) | |
phenol | inhibition of catalase activity | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
H2O2 | catalase activity, pH 8.5, 30°C | Bacillus sp. (in: Bacteria) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
4 * 51000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
204000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | Bacillus sp. (in: Bacteria) | - |
O2 + H2O | - |
? | |
H2O2 | Bacillus sp. (in: Bacteria) 13 | - |
O2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) 13 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
3.8fold from cell culture supernatant | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.94 | - |
purified enzyme, peroxidase activity, mixed-type | Bacillus sp. (in: Bacteria) |
55000 | - |
purified enzyme, catalase activity, mixed-type | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | - |
Bacillus sp. (in: Bacteria) | O2 + H2O | - |
? | |
H2O2 | - |
Bacillus sp. (in: Bacteria) 13 | O2 + H2O | - |
? | |
H2O2 + 2-aminophenol | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
H2O2 + 2-aminophenol | - |
Bacillus sp. (in: Bacteria) 13 | ? | - |
? | |
H2O2 + 3-chlorophenol | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
H2O2 + 3-chlorophenol | - |
Bacillus sp. (in: Bacteria) 13 | ? | - |
? | |
H2O2 + 4-chlorophenol | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
H2O2 + 4-chlorophenol | - |
Bacillus sp. (in: Bacteria) 13 | ? | - |
? | |
H2O2 + 4-nitrophenol | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
H2O2 + dimethylaniline | low activity | Bacillus sp. (in: Bacteria) | ? | - |
? | |
H2O2 + phenol | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
H2O2 + propyl gallate | best peroxidase substrate | Bacillus sp. (in: Bacteria) | ? | - |
? | |
H2O2 + pyrocatechol | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | enzyme also shows alkaline peroxidase activity, which is much lower than the catalase activity, no peroxidase activity with 3-aminophenol, 2,4,6-trichlorophenol, and 3-nitrophenol | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | enzyme also shows alkaline peroxidase activity, which is much lower than the catalase activity, no peroxidase activity with 3-aminophenol, 2,4,6-trichlorophenol, and 3-nitrophenol | Bacillus sp. (in: Bacteria) 13 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 51000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
catalase-peroxidase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
catalase activity | Bacillus sp. (in: Bacteria) |
60 | - |
peroxidase activity | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
85% remaining peroxidase activity after 20 h at pH 9.0 | Bacillus sp. (in: Bacteria) |
60 | - |
pH 8.5, stable up to, catalase and peroxidase activities | Bacillus sp. (in: Bacteria) |
70 | - |
70% decreased activity | Bacillus sp. (in: Bacteria) |
80 | - |
95% decreased activity | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | peroxidase and catalase activities | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | over 80% remaining activity after 1 h at 30°C | Bacillus sp. (in: Bacteria) |
9 | - |
85% remaining activity after 20 h at 30°C | Bacillus sp. (in: Bacteria) |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
4-aminoantipyrine | catalase activity, pH 8.5, 30°C | Bacillus sp. (in: Bacteria) | |
0.14 | - |
phenol | catalase activity, pH 8.5, 30°C | Bacillus sp. (in: Bacteria) |