Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.6 extracted from

  • Ray, M.; Mishra, P.; Das, P.; Sabat, S.C.
    Expression and purification of soluble bio-active rice plant catalase-A from recombinant Escherichia coli (2012), J. Biotechnol., 157, 12-19.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of soluble and inactive His-tagged catalase-A in Escherichia coli strain BL21(DE3) in inclusion bodies. Specific requirement of a thioredoxin fusion partner, the involvement of trigger factor protein and the low temperature treatment during induction period for synthesis of completely solubilized rice plant catalase-A in recombinant Escherichia coli. The bacteria require the supplementation of delta-aminolevulinic acid to produce bio-active recombinant rice catalase-A Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
100
-
H2O2 pH 7.0, 25°C Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
4 * 74000, recombinant His-tagged catalase-A with bound heme cofactor, SDS-PAGE, 4 * 57000-58000, native enzyme Oryza sativa
234000
-
native enzyme, gel filtration Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 H2O2 Oryza sativa
-
O2 + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Oryza sativa A3REN3 CAT-A
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble and inactive His-tagged catalase-A from Escherichia coli strain BL21(DE3) inclusion bodies by EK-affinity chromatography and gel filtration Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 H2O2
-
Oryza sativa O2 + 2 H2O
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 74000, recombinant His-tagged catalase-A with bound heme cofactor, SDS-PAGE, 4 * 57000-58000, native enzyme Oryza sativa

Synonyms

Synonyms Comment Organism
CAT-A
-
Oryza sativa
catalase-A
-
Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Oryza sativa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 40 activity range Oryza sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable up to, a sharp decline in activity above Oryza sativa
55
-
6% activity remaining Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
80000
-
H2O2 pH 7.0, 25°C Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 10 broad optimum Oryza sativa

pH Range

pH Minimum pH Maximum Comment Organism
3 11 activity range Oryza sativa

Cofactor

Cofactor Comment Organism Structure
heme
-
Oryza sativa

General Information

General Information Comment Organism
additional information the enzyme is a mono-functional plant catalase Oryza sativa
physiological function chaperone-specific enzyme regulation, overview Oryza sativa