Cloned (Comment) | Organism |
---|---|
expression of soluble and inactive His-tagged catalase-A in Escherichia coli strain BL21(DE3) in inclusion bodies. Specific requirement of a thioredoxin fusion partner, the involvement of trigger factor protein and the low temperature treatment during induction period for synthesis of completely solubilized rice plant catalase-A in recombinant Escherichia coli. The bacteria require the supplementation of delta-aminolevulinic acid to produce bio-active recombinant rice catalase-A | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
100 | - |
H2O2 | pH 7.0, 25°C | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
4 * 74000, recombinant His-tagged catalase-A with bound heme cofactor, SDS-PAGE, 4 * 57000-58000, native enzyme | Oryza sativa |
234000 | - |
native enzyme, gel filtration | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | Oryza sativa | - |
O2 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | A3REN3 | CAT-A | - |
Purification (Comment) | Organism |
---|---|
recombinant soluble and inactive His-tagged catalase-A from Escherichia coli strain BL21(DE3) inclusion bodies by EK-affinity chromatography and gel filtration | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | - |
Oryza sativa | O2 + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 74000, recombinant His-tagged catalase-A with bound heme cofactor, SDS-PAGE, 4 * 57000-58000, native enzyme | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
CAT-A | - |
Oryza sativa |
catalase-A | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Oryza sativa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 40 | activity range | Oryza sativa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to, a sharp decline in activity above | Oryza sativa |
55 | - |
6% activity remaining | Oryza sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
80000 | - |
H2O2 | pH 7.0, 25°C | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | broad optimum | Oryza sativa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | activity range | Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Oryza sativa |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme is a mono-functional plant catalase | Oryza sativa |
physiological function | chaperone-specific enzyme regulation, overview | Oryza sativa |