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Literature summary for 1.11.1.7 extracted from

  • Lindgren, A.; Ruzgas, T.; Gorton, L.; Csoregi, E.; Bautista Ardila, G.; Sakharov, I.Y.; Gazaryan, I.G.
    Biosensors based on novel peroxidases with improved properties in direct and mediated electron transfer (2000), Biosens. Bioelectron., 15, 491-497.
    View publication on PubMed

Application

Application Comment Organism
medicine biosensors for direct and mediated electron transfer, determination of hydrogen peroxide in RDE mode (mediatorless) Armoracia sp.

Organism

Organism UniProt Comment Textmining
Arachis hypogaea
-
peanut, cationic enzyme
-
Armoracia sp.
-
-
-
Ipomoea batatas
-
sweet potato
-
Nicotiana sylvestris
-
tobacco
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 21% glycosylated Armoracia sp.
glycoprotein 11% glycosylated Nicotiana sylvestris
glycoprotein 20% glycosylated Arachis hypogaea
glycoprotein 16-17% glycosylated Ipomoea batatas

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
78
-
with 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) Arachis hypogaea
1420
-
with 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) Armoracia sp.
1680
-
with 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) Nicotiana sylvestris
5020
-
with guaiacol Ipomoea batatas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2
-
Arachis hypogaea ?
-
?
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2
-
Nicotiana sylvestris ?
-
?
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2
-
Armoracia sp. ?
-
?
guaiacol + H2O2
-
Arachis hypogaea tetraguaiacol + H2O
-
?
guaiacol + H2O2
-
Ipomoea batatas tetraguaiacol + H2O
-
?
guaiacol + H2O2
-
Armoracia sp. tetraguaiacol + H2O
-
?
additional information when the enzyme is immobilized on a graphite electrode, the electrode can substitute the electron donor substrates Armoracia sp. ?
-
?