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Literature summary for 1.11.1.7 extracted from
- Catalytic mechanism of thiol peroxidase from Escherichia coli: Sulfenic acid formation and overoxidation of essential Cys61 (2003), J. Biol. Chem., 278, 9203-9211.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C61S | no activity | Escherichia coli |
| C82S | 72% of wild-type activity | Escherichia coli |
| C82S/C95S | 10% of wild-type activity | Escherichia coli |
| C95S | 20% of wild-type activity | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
cumene hydroperoxide | 25°C, pH 7.0 | Escherichia coli |  |
| 0.023 | - |
thioredoxin 1 | 25°C, pH 7.0 | Escherichia coli | |
| 1.7 | - |
H2O2 | 25°C, pH 7.0 | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17700 | - |
2 * 17700, ESI-mass spectrometry, dimer in solution | Escherichia coli |
| 29500 | - |
sedimentation equilibrium, oxidized enzyme | Escherichia coli |
| 32000 | - |
sedimentation equilibrium, reduced enzyme | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0A862 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, Phenyl-Sepharodse, Q-Sepharose | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| reduced thioredoxin 1 + cumene hydroperoxide | - |
Escherichia coli | oxidized thioredoxin 1 + cumene hydroxide | - |
? | |
| reduced thioredoxin 1 + H2O2 | - |
Escherichia coli | oxidized thioredoxin 1 + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 17700, ESI-mass spectrometry, dimer in solution | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| p20 | - |
Escherichia coli |
| scavengase | - |
Escherichia coli |
| thiol peroxidase | - |
Escherichia coli |
| Tpx | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 70.1 | - |
cumene hydroperoxide | 25°C, pH 7.0 | Escherichia coli | |
| 76 | - |
H2O2 | 25°C, pH 7.0 | Escherichia coli | |
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Release 2023.1 (January 2023)
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