General Stability | Organism |
---|---|
Ca2+-binding increases the protein conformational stability by raising the melting temperature from 67°C to 82°C | Sorghum bicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | SPC4 is activated by Ca2+. Ca2+-binding increases the protein conformational stability by raising the melting temperature from 67°C to 82°C | Sorghum bicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
gel filtration | Sorghum bicolor |
38000 | - |
1 * 38000, SDS-PAGE | Sorghum bicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | P84516 | Moench var. Cauga 10815 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | SPC4 consists of two glycoforms with molecular masses of 34227 Da and 35629 Da. Sugar contents of 3.0% w/w and 6.7% w/w in glycoform I and II, respectively, and a mass of the apoprotein of 33 246 Da. The glycosylation sites of SPC4 are localized in the C-terminus part of the enzyme. Main sugar constituents of the glycan chains are fucose, mannose, xylose, and N-acetylglucosamine | Sorghum bicolor |
Purification (Comment) | Organism |
---|---|
- |
Sorghum bicolor |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Sorghum bicolor | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sorghum bicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferulic acid + H2O2 | - |
Sorghum bicolor | ? | - |
? | |
indole-3-acetic acid + H2O2 | - |
Sorghum bicolor | ? | - |
? | |
N-acetyl-L-tyrosine + H2O2 | - |
Sorghum bicolor | ? | - |
? | |
N-acetyl-L-tyrosine methyl ester + H2O2 | - |
Sorghum bicolor | ? | - |
? | |
p-coumaric acid + H2O2 | - |
Sorghum bicolor | ? | - |
? | |
reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 | - |
Sorghum bicolor | oxidized 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000, SDS-PAGE | Sorghum bicolor |
monomer | 1 * 3428335631, MALDI-TOF-MS | Sorghum bicolor |
Synonyms | Comment | Organism |
---|---|---|
SPC4 | - |
Sorghum bicolor |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
in the absence of added CaCl2, SPC4 readily loses activity when incubated at temperatures above 55°C | Sorghum bicolor |
67 | - |
Ca2+-binding increases the protein conformational stability by raising the melting temperature from 67°C to 82°C | Sorghum bicolor |
82 | - |
Ca2+-binding increases the protein conformational stability by raising the melting temperature from 67°C to 82°C | Sorghum bicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.8 | - |
reaction with reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) | Sorghum bicolor |
5.5 | - |
reaction with ferulic acid | Sorghum bicolor |
6.5 | - |
reaction with N-acetyl-L-tyrosine | Sorghum bicolor |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | 25°C, 2 h, stable | Sorghum bicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the enzyme contains a type-b heme | Sorghum bicolor |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sorghum bicolor | calculated from sequence | - |
11 |