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Literature summary for 1.11.1.7 extracted from

  • Hong, E.S.; Kwon, O.Y.; Ryu, K.
    Strong substrate-stabilizing effect of a water-miscible ionic liquid [BMIM][BF(4)] in the catalysis of horseradish peroxidase (2008), Biotechnol. Lett., 30, 529-533.
    View publication on PubMed
Search for more literature for 1.11.1.7

Inhibitors

Inhibitors Comment Organism Structure
1-butyl-3-methylimidazolium tetrafluoroborate weak, non-competitive inhibitor Armoracia rusticana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8
-
 guaiacol in the absence of 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
4.1
-
 guaiacol in the presence of 5% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
6.8
-
 guaiacol in the presence of 10% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
7.8
-
 guaiacol in the presence of 15% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
15.1
-
 guaiacol in the presence of 20% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
22.5
-
 guaiacol in the presence of 25% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana

Organism

Organism UniProt Comment Textmining
Armoracia rusticana
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guaiacol + H2O2
-
 Armoracia rusticana tetraguaiacol + H2O
-
 ?

Synonyms

Synonyms Comment Organism
horseradish peroxidase
-
 Armoracia rusticana
HRP
-
 Armoracia rusticana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.7
-
 guaiacol in the presence of 25% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
9.3
-
 guaiacol in the presence of 20% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
10.6
-
 guaiacol in the presence of 15% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
10.9
-
 guaiacol in the presence of 10% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
12.2
-
 guaiacol in the absence of 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana
12.4
-
 guaiacol in the presence of 5% (v/v) 1-butyl-3-methylimidazolium tetrafluoroborate, in potassium phosphate buffer (20 mM, pH 7.0) at 25°C Armoracia rusticana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2900
-
 1-butyl-3-methylimidazolium tetrafluoroborate at 25°C Armoracia rusticana