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Literature summary for 1.11.1.7 extracted from
- HTHP: a novel class of hexameric, tyrosine-coordinated heme proteins (2007), J. Mol. Biol., 368, 1122-1131.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta (DE3) | Ruegeria pomeroyi DSS-3 |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Ruegeria pomeroyi DSS-3 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | peroxidase activity is not inhibited by 3-amino-1,2,4-triazole concentrations up to 20 mM | Ruegeria pomeroyi DSS-3 |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 8300 | - |
ring-like arrangement of six monomers, 6 * 7900 (SDS-PAGE), 6 * 8300 calculated | Ruegeria pomeroyi DSS-3 |
| 54000 | - |
gel filtration | Ruegeria pomeroyi DSS-3 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ruegeria pomeroyi DSS-3 | Q5LLG6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified to homogeneity as holoprotein by affinity chromatography | Ruegeria pomeroyi DSS-3 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity with o-dianisidine is 10fold lower at pH 7.5 and not measurable at higher pH values due to its instability in alkaline solution | Ruegeria pomeroyi DSS-3 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no peroxidase activity is observed with ascorbate as substrate | Ruegeria pomeroyi DSS-3 | ? | - |
? |  |
| NADH + H2O2 | - |
Ruegeria pomeroyi DSS-3 | NAD+ + H2O | - |
? | |
| NADPH + H2O2 | - |
Ruegeria pomeroyi DSS-3 | NADP+ + H2O | - |
? | |
| o-dianisidine + H2O2 | - |
Ruegeria pomeroyi DSS-3 | ? + H2O | - |
? | |
| pyrogallol + H2O2 | - |
Ruegeria pomeroyi DSS-3 | purpurogallin + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | ring-like arrangement of six monomers, 6 * 7900 (SDS-PAGE), 6 * 8300 calculated | Ruegeria pomeroyi DSS-3 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HTHP | peroxidase and catalase activity | Ruegeria pomeroyi DSS-3 |
| tyrosine-coordinated heme protein | - |
Ruegeria pomeroyi DSS-3 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
maximal activity | Ruegeria pomeroyi DSS-3 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
high thermal stability. HTHP is stable under a wide range of temperatures. Thermal unfolding is measured up to 110°C and in the presence of high concentrations of guanidinium hydrochloride. The melting point of HTHP is estimated to be around 130°C. Catalatic activity is tested after incubation for 10 min at 85°C and 90°C and is found to be reduced by less than 5% under both conditions | Ruegeria pomeroyi DSS-3 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
at physiological pH values the highest activity is detectable with NADH or NADPH as substrates (3.0 U/mg at pH 8.7) | Ruegeria pomeroyi DSS-3 |
| 11.7 | - |
highest peroxidase activity (25.7 U/mg), with pyrogallol as substrate | Ruegeria pomeroyi DSS-3 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
high thermal stability. HTHP is stable under a wide range of pH | Ruegeria pomeroyi DSS-3 |
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Release 2023.1 (January 2023)
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