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Literature summary for 1.11.2.1 extracted from
- Crystallization of a 45 kDa peroxygenase/peroxidase from the mushroom Agrocybe aegerita and structure determination by SAD utilizing only the haem iron (2010), Acta Crystallogr. Sect. F, 66, 693-698.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using ammonium sulfate as the precipitant a total of three suitable crystal forms are obtained at different pH values (PIIpH46 with 2.0 M ammonium sulfate in 100 mM TrisHCl pH 8.5, PIIpH56 with 2.4 M ammonium sulfate in 100 mM sodium citrate pH 5.6, and PIIpH85 with 2.0 M ammonium sulfate in 200 mM sodium acetate pH 4.6) | Cyclocybe aegerita |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
SDS-PAGE | Cyclocybe aegerita |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyclocybe aegerita | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | highly glycosylated peroxygenase | Cyclocybe aegerita |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| SP Sepharose column chromatography, Mono Q column chromatography, and Mono S column chromatography | Cyclocybe aegerita |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| veratryl alcohol + H2O2 + H+ | - |
Cyclocybe aegerita | veratraldehyde + H2O | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 45 kDa peroxygenase/peroxidase | - |
Cyclocybe aegerita |
| APO | - |
Cyclocybe aegerita |
| aromatic peroxygenase | - |
Cyclocybe aegerita |
| PII | - |
Cyclocybe aegerita |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Cyclocybe aegerita | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Cyclocybe aegerita | the enzyme has two pI-values of 5.6 and 5.2, isoelectric focusing | - |
5.2 |
| Cyclocybe aegerita | the enzyme has two pI-values of 5.6 and 5.2, isoelectric focusing | - |
5.6 |
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Release 2023.1 (January 2023)
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