Literature summary for 1.11.2.1 extracted from

Kluge, M.G.; Ullrich, R.; Scheibner, K.; Hofrichter, M.
Spectrophotometric assay for detection of aromatic hydroxylation catalyzed by fungal haloperoxidase-peroxygenase (2007), Appl. Microbiol. Biotechnol., 75, 1473-1478.

Search for more literature for 1.11.2.1

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.037	-	2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)	in sodium citrate buffer, pH 4.5, at 25°C	Cyclocybe aegerita
0.298	-	2,6-dimethoxyphenol	in sodium citrate buffer, pH 7.0, at 25°C	Cyclocybe aegerita
0.32	-	Naphthalene	in potassium phosphate buffer, pH 7.0, at 25°C	Cyclocybe aegerita
1.001	-	benzyl alcohol	in sodium citrate buffer, pH 7.0, at 25°C	Cyclocybe aegerita
1.313	-	H2O2	in sodium citrate buffer, pH 7.0, at 25°C	Cyclocybe aegerita
2.367	-	veratryl alcohol	in sodium citrate buffer, pH 7.0, at 25°C	Cyclocybe aegerita

Organism

Organism	UniProt	Comment	Textmining
Cyclocybe aegerita	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
fast protein liquid chromatography	Cyclocybe aegerita

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
74.8	-	purified enzyme, using veratryl alcohol as substrate, at pH 7.0 and 25°C	Cyclocybe aegerita

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 2,6-dimethoxyphenol + 2 H2O2	-	Cyclocybe aegerita	cerulignone + 2 H2O	-	?
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2	-	Cyclocybe aegerita	?	-	?
benzyl alcohol + H2O2	-	Cyclocybe aegerita	benzaldehyde + H2O	-	?
naphthalene + H2O2	-	Cyclocybe aegerita	1-naphthol + H2O	in addition to 1-naphthol a smaller amount of naphthalene is converted to 2 naphthol dependent on pH, at pH 7.0-8.0 3% 2-naphthol and 97% 1-naphthol are formed while at pH 3.0 18% 2-naphthol and 82% 1-naphthol are formed. Traces of 1-naphthol are later oxidized to 1,4-naphthoquinone	?
veratryl alcohol + H2O2 + H+	-	Cyclocybe aegerita	veratraldehyde + H2O	-	?

Synonyms

Synonyms	Comment	Organism
Agrocybe aegerita peroxidase	P II, main isoform of Agrocybe aegerita peroxidase	Cyclocybe aegerita
haloperoxidase-peroxygenase	-	Cyclocybe aegerita

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
85	-	veratryl alcohol	in sodium citrate buffer, pH 7.0, at 25°C	Cyclocybe aegerita
108	-	2,6-dimethoxyphenol	in sodium citrate buffer, pH 7.0, at 25°C	Cyclocybe aegerita
166	-	Naphthalene	in potassium phosphate buffer, pH 7.0, at 25°C	Cyclocybe aegerita
269	-	benzyl alcohol	in sodium citrate buffer, pH 7.0, at 25°C	Cyclocybe aegerita
283	-	2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)	in sodium citrate buffer, pH 4.5, at 25°C	Cyclocybe aegerita
367	-	H2O2	in sodium citrate buffer, pH 7.0, at 25°C	Cyclocybe aegerita

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	8	activity related to 1-naphthol formation shows a broad pH optimum between 5.0 and 8.0	Cyclocybe aegerita

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
3	9	substantial oxygenation of naphthalene still occurs at pH 3.0 and pH 9.0 (60 and 70% of the maximum conversion rate, respectively)	Cyclocybe aegerita

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Cyclocybe aegerita

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
35.8	-	veratryl alcohol	in sodium citrate buffer, pH 7.0, at 25°C	Cyclocybe aegerita
269	-	benzyl alcohol	in sodium citrate buffer, pH 7.0, at 25°C	Cyclocybe aegerita
279	-	H2O2	in sodium citrate buffer, pH 7.0, at 25°C	Cyclocybe aegerita
361	-	2,6-dimethoxyphenol	in sodium citrate buffer, pH 7.0, at 25°C	Cyclocybe aegerita
517	-	Naphthalene	in potassium phosphate buffer, pH 7.0, at 25°C	Cyclocybe aegerita
7670	-	2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)	in sodium citrate buffer, pH 4.5, at 25°C	Cyclocybe aegerita

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Release 2023.1 (January 2023)