Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Cyclocybe aegerita | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
SDS-PAGE | Cyclocybe aegerita |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyclocybe aegerita | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Cyclocybe aegerita |
Purification (Comment) | Organism |
---|---|
ultrafiltration, SP Sepharose column chromatography, and Mono P column chromatography | Cyclocybe aegerita |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
77 | - |
major enzyme form P I, after 33fold purification, using veratryl alchol as substrate, pH and temperature not specified in the publication | Cyclocybe aegerita |
94 | - |
major enzyme form P II, after 40fold purification, using veratryl alchol as substrate, pH and temperature not specified in the publication | Cyclocybe aegerita |
103 | - |
major enzyme form P III, after 44fold purification, using veratryl alchol as substrate, pH and temperature not specified in the publication | Cyclocybe aegerita |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
naphthalene + H2O2 | naphthalene is regioselectively converted into 1-naphthol and 2-naphthol at a ratio of 12:1 | Cyclocybe aegerita | 1-naphthol + 2-naphthol + H2O | - |
? | |
toluene + H2O2 | all peroxygenase fractions oxygenate toluene at both the side chain and the aromatic ring with a ratio of side chain versus aromatic hydroxylation of about 2:1 in all cases | Cyclocybe aegerita | benzyl alcohol + benzaldehyde + benzoic acid + 2-cresol + 4-cresol + methylhydroquinone + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | - |
Cyclocybe aegerita | veratraldehyde + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aromatic peroxygenase | - |
Cyclocybe aegerita |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cyclocybe aegerita | the final protein fraction had a molecular mass of 46 kDa but still consists of several incompletely separated proteins with slightly differing isoelectric points (pI 5.2, 5.6, 6.1), probably representing differently glycosylated isoforms | - |
5.2 |
Cyclocybe aegerita | the final protein fraction had a molecular mass of 46 kDa but still consists of several incompletely separated proteins with slightly differing isoelectric points (pI 5.2, 5.6, 6.1), probably representing differently glycosylated isoforms | - |
5.6 |
Cyclocybe aegerita | the final protein fraction had a molecular mass of 46 kDa but still consists of several incompletely separated proteins with slightly differing isoelectric points (pI 5.2, 5.6, 6.1), probably representing differently glycosylated isoforms | - |
6.1 |