Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetone | 2% (v/v) acetone boosts the activity of the enzyme by up to 2fold | Hypoxylon sp. EC38 | |
acetonitrile | 5% (v/v) acetonitrile boosts the activity of the enzyme by up to 6fold | Hypoxylon sp. EC38 |
Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Hypoxylon sp. EC38 |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method | Hypoxylon sp. EC38 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DMSO | DMSO is hardly tolerated by the enzyme (the residual activity drops to near 0% with 2% (v/v) DMSO) | Hypoxylon sp. EC38 | |
H2O2 | the enzyme retains 75% and 40% of the activity in the presence of 5 mM and 10 mM H2O2 whereas the optimal activity is observed at 2 mM H2O2 | Hypoxylon sp. EC38 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
5-nitro-1,3-benzodioxole | at pH 7.0 and 25°C | Hypoxylon sp. EC38 | |
0.03 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | at pH 4.5 and 25°C | Hypoxylon sp. EC38 | |
0.054 | - |
indole | at pH 7.0 and 25°C | Hypoxylon sp. EC38 | |
0.0575 | - |
2,6-dimethoxyphenol | at pH 5.0 and 25°C | Hypoxylon sp. EC38 | |
0.68 | - |
benzyl alcohol | at pH 7.0 and 25°C | Hypoxylon sp. EC38 | |
0.758 | - |
3,4-dimethoxybenzyl alcohol | at pH 4.0 and 25°C | Hypoxylon sp. EC38 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hypoxylon sp. EC38 | A0A1Y2TH07 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Hypoxylon sp. EC38 |
Purification (Comment) | Organism |
---|---|
CaptoQ column chromatography and His-Trap column chromatography | Hypoxylon sp. EC38 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chloro-4-ethenylbenzene + H2O2 | - |
Hypoxylon sp. EC38 | (2R)-2-(4-chlorophenyl)oxirane + H2O | - |
? | |
1-phenylethanol + H2O2 | - |
Hypoxylon sp. EC38 | 1-phenylethanone + H2O | - |
? | |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Hypoxylon sp. EC38 | oxidized 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
2,6-dimethoxyphenol + H2O2 | - |
Hypoxylon sp. EC38 | ? | - |
? | |
3,4-dimethoxybenzyl alcohol + H2O2 | - |
Hypoxylon sp. EC38 | 3,4-dimethoxybenzaldehyde + H2O | - |
? | |
5-nitro-1,3-benzodioxole + H2O2 | - |
Hypoxylon sp. EC38 | 4-nitrocatechol + formic acid + H2O | - |
? | |
benzyl alcohol + H2O2 | - |
Hypoxylon sp. EC38 | benzaldehyde + H2O | - |
? | |
cyclohexene + H2O2 | - |
Hypoxylon sp. EC38 | cyclohexene oxide + H2O | - |
? | |
ethenylbenzene + H2O2 | - |
Hypoxylon sp. EC38 | (2S)-2-phenyloxirane + H2O | - |
? | |
ethylbenzene + H2O2 | 39% conversion | Hypoxylon sp. EC38 | (1R)-1-phenylethanol + acetophenone + H2O | - |
? | |
indole + H2O2 | - |
Hypoxylon sp. EC38 | ? | - |
? | |
indole + H2O2 | - |
Hypoxylon sp. EC38 | indigo + H2O | - |
? | |
naphthalene + H2O2 | - |
Hypoxylon sp. EC38 | ? | - |
? | |
veratryl alcohol + H2O2 | - |
Hypoxylon sp. EC38 | 3,4-dimethoxybenzaldehyde + 3,4-dimethoxybenzoic acid + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE | Hypoxylon sp. EC38 |
Synonyms | Comment | Organism |
---|---|---|
UPO | - |
Hypoxylon sp. EC38 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 50 | at 35°C, there is some activity loss after 60 min but the enzyme remains mostly active even after longer incubations. The enzyme is mostly inactivated after 20-30 minute exposures to temperatures higher than 50°C. Conversely, the enzyme overall activity is minimally affected at 40°C and after 90 min the enzyme mostly loses activity | Hypoxylon sp. EC38 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.89 | - |
indole | at pH 7.0 and 25°C | Hypoxylon sp. EC38 | |
4.56 | - |
benzyl alcohol | at pH 7.0 and 25°C | Hypoxylon sp. EC38 | |
7.15 | - |
3,4-dimethoxybenzyl alcohol | at pH 4.0 and 25°C | Hypoxylon sp. EC38 | |
8 | - |
H2O2 | at pH 4.5 and 25°C | Hypoxylon sp. EC38 | |
10.4 | - |
5-nitro-1,3-benzodioxole | at pH 7.0 and 25°C | Hypoxylon sp. EC38 | |
11 | - |
2,6-dimethoxyphenol | at pH 5.0 and 25°C | Hypoxylon sp. EC38 | |
17.29 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | at pH 4.5 and 25°C | Hypoxylon sp. EC38 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
pH 4.0 is optimal for the conversions of 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) and 3,4-dimethoxybenzyl alcohol | Hypoxylon sp. EC38 |
5 | - |
pH 5.0 is optimal for the conversion of 2,6-dimethoxyphenol | Hypoxylon sp. EC38 |
7 | - |
the enzyme is optimally active at neutral pH with indole, 5-nitro-1,3-benzodioxole, and benzyl alcohol | Hypoxylon sp. EC38 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Hypoxylon sp. EC38 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
benzyl alcohol | at pH 7.0 and 25°C | Hypoxylon sp. EC38 | |
10 | - |
3,4-dimethoxybenzyl alcohol | at pH 4.0 and 25°C | Hypoxylon sp. EC38 | |
16 | - |
indole | at pH 7.0 and 25°C | Hypoxylon sp. EC38 | |
191 | - |
2,6-dimethoxyphenol | at pH 5.0 and 25°C | Hypoxylon sp. EC38 | |
570 | - |
5-nitro-1,3-benzodioxole | at pH 7.0 and 25°C | Hypoxylon sp. EC38 | |
570 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | at pH 4.5 and 25°C | Hypoxylon sp. EC38 |