Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 (pREP4) cells | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
ferric P450BSbeta in the substrate-bound form, sitting drop vapor diffusion method, using 10% (w/v) polyethylene glycol 3350 and 50 mM MES at pH 6.8, at 20°C | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
F79L | the specific activity is reduced by half compared to the wild type enzyme | Bacillus subtilis |
F79L/V170F | the double mutant exhibits 10% of the activity of the wild type enzyme | Bacillus subtilis |
V170F | the mutant exhibits 30% of the activity of the wild type enzyme | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacillus subtilis | P450BSbeta produces both the beta-OH (60%) and the alpha-OH (40%) fatty acids. Ferredoxin, ferredoxin reductase, and P450 reductase systems do not appear to function in P450BSbeta reactions. P450BSbeta does not require any electrons and protons for catalytic activity, because it utilizes H2O2 as an oxidant instead of O2/2e-/2H+. This enzyme requires neither a reductase nor a proton delivery system | ? | - |
? | |
myristic acid + H2O2 | Bacillus subtilis | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O31440 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | P450BSbeta produces both the beta-OH (60%) and the alpha-OH (40%) fatty acids. Ferredoxin, ferredoxin reductase, and P450 reductase systems do not appear to function in P450BSbeta reactions. P450BSbeta does not require any electrons and protons for catalytic activity, because it utilizes H2O2 as an oxidant instead of O2/2e-/2H+. This enzyme requires neither a reductase nor a proton delivery system | Bacillus subtilis | ? | - |
? | |
myristic acid + H2O2 | - |
Bacillus subtilis | ? | - |
? | |
palmitic acid + H2O2 | - |
Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP152A1 | - |
Bacillus subtilis |
P450BSbeta | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Bacillus subtilis |