Cloned (Comment) | Organism |
---|---|
heterologous expression in Ralstonia eutropha | Rhodococcus opacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus opacus | - |
- |
- |
Rhodococcus opacus MR11 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.7 | - |
recombinant enzyme expressed in Ralstonia eutropha | Rhodococcus opacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + NAD+ | - |
Rhodococcus opacus | H+ + NADH | - |
r | |
H2 + NAD+ | - |
Rhodococcus opacus MR11 | H+ + NADH | - |
r | |
H2 + oxidized benzyl viologen | - |
Rhodococcus opacus | H+ + reduced benzyl viologen | - |
r | |
H2 + oxidized benzyl viologen | - |
Rhodococcus opacus MR11 | H+ + reduced benzyl viologen | - |
r | |
oxidized benzyl viologen + NADH | - |
Rhodococcus opacus | reduced benzyl viologen + NAD+ | - |
? | |
oxidized benzyl viologen + NADH | - |
Rhodococcus opacus MR11 | reduced benzyl viologen + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | in low-salt buffer the tetrameric enzyme dissociates into 2 dimeric forms accompanied by the failure to reduce NAD+ | Rhodococcus opacus |