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Literature summary for 1.12.1.2 extracted from

  • Loescher, S.; Burgdorf, T.; Buhrke, T.; Friedrich, B.; Dau, H.; Haumann, M.
    Non-standard structures of the Ni-Fe cofactor in the regulatory and the NAD-reducing hydrogenases from Ralstonia eutropha (2005), Biochem. Soc. Trans., 33, 25-27.
    View publication on PubMed
Search for more literature for 1.12.1.2

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by O2 Cupriavidus necator

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Cupriavidus necator
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
CN- enzyme contains four cyanides in its active site, the Ni2+ bound one is responsible for the insensitivity towards oxygen, the active site is a (enzyme-Cys)2(CN)Ni(micro-enzyme-Cys)2Fe(CN)3(CO) centre, the CN- bound to the nickel ion can be irreversibly removed inducing enzyme inhibition by oxygen Cupriavidus necator
CO bound to the active site, the active site is a (enzyme-Cys)2(CN)Ni(micro-enzyme-Cys)2Fe(CN)3(CO) centre Cupriavidus necator
Fe2+ the active site is a (enzyme-Cys)2(CN)Ni(micro-enzyme-Cys)2Fe(CN)3(CO) centre Cupriavidus necator
Ni2+ the active site is a (enzyme-Cys)2(CN)Ni(micro-enzyme-Cys)2Fe(CN)3(CO) centre, H2 activation solely takes place on Ni2+ Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2 + NAD+ Cupriavidus necator enzyme provides reducing equivalents for CO2 fixation H+ + NADH
-
 r
additional information Cupriavidus necator enzyme regulation involves a regulatory hydrogenase RH which acts as a sensor for H2 content, interaction via signal cascade ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + NAD+
-
 Cupriavidus necator H+ + NADH
-
 r
H2 + NAD+ enzyme provides reducing equivalents for CO2 fixation Cupriavidus necator H+ + NADH
-
 r
additional information enzyme regulation involves a regulatory hydrogenase RH which acts as a sensor for H2 content, interaction via signal cascade Cupriavidus necator ?
-
 ?

Synonyms

Synonyms Comment Organism
NAD-reducing hydrogenase
-
 Cupriavidus necator
SH
-
 Cupriavidus necator
soluble hydrogenase
-
 Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
additional information Ni-Fe cofactor, analysis of the non-standard structure of the Ni-Fe cofactor bound at the active site, interaction mechanism with H2, structural changes during reaction, reduction, and inactivation, the cofactor structure is (enzyme-Cys)2(CN)Ni(micro-enzyme-Cys)2Fe(CN)3(CO), overview Cupriavidus necator
NAD+
-
 Cupriavidus necator
NADH
-
 Cupriavidus necator