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Literature summary for 1.12.1.2 extracted from

  • Mohapatra, A.; Leul, M.; Mattsson, U.; Sellstedt, A.
    A hydrogen-evolving enzyme is present in Frankia sp. R43 (2004), FEMS Microbiol. Lett., 236, 235-240.
    View publication on PubMed
Search for more literature for 1.12.1.2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2 + NAD+ Frankia sp. R43 H2 producing activity is not dependent on NAD+ reduction H+ + NADH
-
 r
H2 + oxidized methyl viologen Frankia sp. R43 the artificial electron donor is accepted by the cells for H2 production when added to the culture medium H+ + reduced methyl viologen
-
 r
additional information Frankia sp. R43 enzyme is the key enzyme in hydrogen metabolism, no activity in strain R43 under aerobic nitrogen-limited conditions ?
-
 ?

Organism

Organism UniProt Comment Textmining
Frankia sp. R43 P22317 hoxF, alpha-subunit; LLR02022, nitrogen-fixing actinomycete, no activity in strain KB5
-
Frankia sp. R43 P22318 hoxU, gamma-subunit; LLR02022, nitrogen-fixing actinomycete, no activity in strain KB5
-
Frankia sp. R43 P22319 hoxY, delta-subunit; LLR02022, nitrogen-fixing actinomycete, no activity in strain KB5
-
Frankia sp. R43 P22320 hoxH, beta-subunit; LLR02022, nitrogen-fixing actinomycete, no activity in strain KB5
-

Source Tissue

Source Tissue Comment Organism Textmining
hypha
-
 Frankia sp. R43
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 H2-uptake activity at different conditions Frankia sp. R43
0.0006
-
 cell culture in nitrogen-enriched medium, anaerobic conditions, H2 production using reduced methyl viologen as electron donor Frankia sp. R43
0.0018
-
 cell culture in nitrogen-limited medium, anaerobic conditions, H2 production using reduced methyl viologen as electron donor Frankia sp. R43

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + NAD+
-
 Frankia sp. R43 H+ + NADH
-
 r
H2 + NAD+ H2 producing activity is not dependent on NAD+ reduction Frankia sp. R43 H+ + NADH
-
 r
H2 + oxidized methyl viologen
-
 Frankia sp. R43 H+ + reduced methyl viologen
-
 r
H2 + oxidized methyl viologen the artificial electron donor is accepted by the cells for H2 production when added to the culture medium Frankia sp. R43 H+ + reduced methyl viologen
-
 r
additional information enzyme is the key enzyme in hydrogen metabolism, no activity in strain R43 under aerobic nitrogen-limited conditions Frankia sp. R43 ?
-
 ?

Synonyms

Synonyms Comment Organism
hydrogen-evolving enzyme
-
 Frankia sp. R43

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
 assay at, free living cell culture in vivo Frankia sp. R43

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at, free living cell culture in vivo Frankia sp. R43

Cofactor

Cofactor Comment Organism Structure
additional information methyl viologen can replace NAD+/NADH Frankia sp. R43
NAD+
-
 Frankia sp. R43
NADH
-
 Frankia sp. R43