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Literature summary for 1.12.1.2 extracted from
- Characterization of catalytic properties of hydrogenase isolated from the unicellular cyanobacterium Gloeocapsa alpicola CALU 743 (2006), Biochemistry, 71, 1370-1376.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0007 | - |
NADPH | 30°C, pH 7.0 | Gloeocapsa alpicola |  |
| 0.0023 | - |
NADH | 30°C, pH 7.0 | Gloeocapsa alpicola | |
| 0.0035 | - |
NAD+ | 30°C, pH 8.5 | Gloeocapsa alpicola | |
| 0.0306 | - |
reduced methyl viologen | 30°C, pH 7.0 | Gloeocapsa alpicola | |
| 0.0375 | - |
oxidized methyl viologen | 30°C, pH 8.5 | Gloeocapsa alpicola | |
| 0.0578 | - |
Oxidized benzyl viologen | 30°C, pH 8.5 | Gloeocapsa alpicola | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Gloeocapsa alpicola | physiological role of the hydrogenase seems to be equally associated with both formation and uptake of H2, depending on the redox state of the intracellular medium | ? | - |
? | |
| additional information | Gloeocapsa alpicola CALU 743 | physiological role of the hydrogenase seems to be equally associated with both formation and uptake of H2, depending on the redox state of the intracellular medium | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gloeocapsa alpicola | - |
- |
- |
| Gloeocapsa alpicola CALU 743 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gloeocapsa alpicola |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.8 | - |
- |
Gloeocapsa alpicola |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + NAD+ | only 3-8% of the benzyl biologen or methyl viologen-dependent values. The enzyme interacts with NADP+ and NADPH, but is more specific to NAD+ and NADH | Gloeocapsa alpicola | H+ + NADH | - |
r | |
| H2 + NAD+ | only 3-8% of the benzyl biologen or methyl viologen-dependent values. The enzyme interacts with NADP+ and NADPH, but is more specific to NAD+ and NADH | Gloeocapsa alpicola CALU 743 | H+ + NADH | - |
r | |
| H2 + NADP+ | only 3-8% of the benzyl biologen or methyl viologen-dependent values. The enzyme interacts with NADP+ and NADPH, but is more specific to NAD+ and NADH | Gloeocapsa alpicola | H+ + NADPH | - |
r | |
| H2 + NADP+ | only 3-8% of the benzyl biologen or methyl viologen-dependent values. The enzyme interacts with NADP+ and NADPH, but is more specific to NAD+ and NADH | Gloeocapsa alpicola CALU 743 | H+ + NADPH | - |
r | |
| H2 + oxidized benzyl viologen | - |
Gloeocapsa alpicola | reduced benzyl viologen + H+ | - |
? | |
| H2 + oxidized benzyl viologen | - |
Gloeocapsa alpicola CALU 743 | reduced benzyl viologen + H+ | - |
? | |
| H2 + oxidized methyl viologen | - |
Gloeocapsa alpicola | reduced methyl viologen + H+ | - |
r | |
| H2 + oxidized methyl viologen | - |
Gloeocapsa alpicola CALU 743 | reduced methyl viologen + H+ | - |
r | |
| additional information | physiological role of the hydrogenase seems to be equally associated with both formation and uptake of H2, depending on the redox state of the intracellular medium | Gloeocapsa alpicola | ? | - |
? | |
| additional information | physiological role of the hydrogenase seems to be equally associated with both formation and uptake of H2, depending on the redox state of the intracellular medium | Gloeocapsa alpicola CALU 743 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Hox type hydrogenase | - |
Gloeocapsa alpicola |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
evolution of H2 in the presence of NADH or NADPH | Gloeocapsa alpicola |
| 50 | - |
evolution of H2 in the presence of NADH or NADPH | Gloeocapsa alpicola |
| 65 | - |
evolution of H2 in the presence of reduced methyl viologen | Gloeocapsa alpicola |
| 70 | - |
evolution of H2 in the presence of methyl viologen or benzyl viologen | Gloeocapsa alpicola |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | - |
evolution of H2 in the presence of reduced methyl viologen | Gloeocapsa alpicola |
| 6.8 | - |
evolution of H2 in the presence of NADH or NADPH | Gloeocapsa alpicola |
| 8.5 | - |
evolution of H2 in the presence of NADH or NADPH | Gloeocapsa alpicola |
| 9 | - |
evolution of H2 in the presence of methyl viologen or benzyl viologen | Gloeocapsa alpicola |
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