Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | product inhibition; product inhibition | Cupriavidus necator | |
NADH | product inhibition; product inhibition | Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
NADH | pH 8.0, temperature not specified in the publication | Cupriavidus necator | |
0.197 | - |
NAD+ | pH 8.0, temperature not specified in the publication | Cupriavidus necator |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | P22317 | subunit alpha | - |
Cupriavidus necator | P22318 | subunit gamma | - |
Cupriavidus necator DSM 428 | P22317 | subunit alpha | - |
Cupriavidus necator DSM 428 | P22318 | subunit gamma | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H+ + NADH | module HoxFU shows clear electrocatalytic activity for both NAD+ reduction and NADH oxidation with minimal overpotential relative to the potential of the NAD+/NADH couple | Cupriavidus necator | H2 + NAD+ | - |
r | |
H+ + NADH | module HoxFU shows clear electrocatalytic activity for both NAD+ reduction and NADH oxidation with minimal overpotential relative to the potential of the NAD+/NADH couple | Cupriavidus necator DSM 428 | H2 + NAD+ | - |
r | |
H2 + NAD+ | module HoxFU shows clear electrocatalytic activity for both NAD+ reduction and NADH oxidation with minimal overpotential relative to the potential of the NAD+/NADH couple | Cupriavidus necator | H+ + NADH | - |
r | |
H2 + NAD+ | module HoxFU shows clear electrocatalytic activity for both NAD+ reduction and NADH oxidation with minimal overpotential relative to the potential of the NAD+/NADH couple | Cupriavidus necator DSM 428 | H+ + NADH | - |
r | |
additional information | in protein film electrochemical experiments the electrocatalytic current is unaffected by O2. In aerobic solution assays, a moderate superoxide production rate of 54 nmol per mg of protein is observed, meaning that the formation of reactive oxygen species observed for the native enzyme can be attributed mainly to module HoxFU | Cupriavidus necator | ? | - |
? | |
additional information | in protein film electrochemical experiments the electrocatalytic current is unaffected by O2. In aerobic solution assays, a moderate superoxide production rate of 54 nmol per mg of protein is observed, meaning that the formation of reactive oxygen species observed for the native enzyme can be attributed mainly to module HoxFU | Cupriavidus necator DSM 428 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HoxF | - |
Cupriavidus necator |
HoxU | - |
Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | module HoxFU accommodates a [2Fe2S] cluster, FMN and a series of [4Fe4S] clusters | Cupriavidus necator | |
[2Fe-2S]-center | HoxFU accommodates a [2Fe2S] cluster, FMN and a series of [4Fe4S] clusters | Cupriavidus necator | |
[4Fe-4S]-center | HoxFU accommodates a [2Fe2S] cluster, FMN and a series of [4Fe4S] clusters | Cupriavidus necator |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
NAD+ | pH 8.0, temperature not specified in the publication | Cupriavidus necator | |
0.2 | - |
NADH | pH 8.0, temperature not specified in the publication | Cupriavidus necator |