Literature summary for 1.12.1.3 extracted from

De Luca, G.; De Philip, P.; Rousset, M.; Belaich, J.P.; Dermoun, Z.
The NADP-reducing hydrogenase of Desulfovibrio fructosovorans: evidence for a native complex with hydrogen-dependent methyl-viologen-reducing activity (1998), Biochem. Biophys. Res. Commun., 248, 591-596.

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Cloned(Commentary)

Cloned (Comment)	Organism
HndA and HndB subunit	Solidesulfovibrio fructosivorans

General Stability

General Stability	Organism
50% loss of enzyme activity when extracts are exposed for 15 min to air	Solidesulfovibrio fructosivorans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.09	-	NADP+	-	Solidesulfovibrio fructosivorans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
H2 + NADP+	Solidesulfovibrio fructosivorans	biological function probably involves production of reduced pyridine nucleotides	H+ + NADPH	-	?

Organism

Organism	UniProt	Comment	Textmining
Solidesulfovibrio fructosivorans	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2 + NADP+	-	Solidesulfovibrio fructosivorans	H+ + NADPH	-	?
H2 + NADP+	biological function probably involves production of reduced pyridine nucleotides	Solidesulfovibrio fructosivorans	H+ + NADPH	-	?
H2 + oxidized methyl viologen	reduction requires all four subunits, a form lacking HndD shows no activity towards methyl viologen	Solidesulfovibrio fructosivorans	H+ + reduced methyl viologen	-	?

Subunits

Subunits	Comment	Organism
tetramer	four different subunits with the following molecular weights: HndA 19000, HndB 14000, HndC 52000, HndD 64000, SDS-PAGE	Solidesulfovibrio fructosivorans
tetramer	four different subunits with the following molecular weights: HndA 18800, HndB 13800, HndC 52000, HndD 63400, SDS-PAGE	Solidesulfovibrio fructosivorans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Solidesulfovibrio fructosivorans

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Release 2023.1 (January 2023)