Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | - |
Desulfovibrio desulfuricans | |
KI | above 0.5 M, considerable inhibition of H2 evolution | Desulfovibrio desulfuricans | |
N-bromosuccinimide | 0.07 mM, complete loss of activity | Desulfovibrio desulfuricans | |
NaCl | above 0.5 M, considerable inhibition of H2 evolution | Desulfovibrio desulfuricans | |
NaF | above 0.5 M, considerable inhibition of H2 evolution | Desulfovibrio desulfuricans | |
O2 | strong inhibition of H2 evolution from reduced methyl viologen | Desulfovibrio desulfuricans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
ferrocytochrome c3 | H2-evolution assay | Desulfovibrio desulfuricans | |
0.003 | - |
reduced methyl viologen | H2-evolution assay | Desulfovibrio desulfuricans | |
0.08 | - |
reduced benzyl viologen | H2-evolution assay | Desulfovibrio desulfuricans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound to | Desulfovibrio desulfuricans | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains 6 iron atoms and six acid-labile sulfur groups per molecule, presence of [4Fe-4S]3+ or [3Fe-3S]3+ | Desulfovibrio desulfuricans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
gel filtration | Desulfovibrio desulfuricans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Desulfovibrio desulfuricans | cytochrome c3 is the natural electron acceptor | ? | - |
? | |
additional information | Desulfovibrio desulfuricans Norway | cytochrome c3 is the natural electron acceptor | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfovibrio desulfuricans | - |
Norway | - |
Desulfovibrio desulfuricans Norway | - |
Norway | - |
Purification (Comment) | Organism |
---|---|
membrane-bound enzyme | Desulfovibrio desulfuricans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Desulfovibrio desulfuricans |
Storage Stability | Organism |
---|---|
4°C, atmosphere of N2, air or H2, very little loss of activity after 4 weeks | Desulfovibrio desulfuricans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H+ + neutral red | - |
Desulfovibrio desulfuricans | H2 + oxidized neutral red | - |
? | |
H+ + neutral red | - |
Desulfovibrio desulfuricans Norway | H2 + oxidized neutral red | - |
? | |
H+ + phenosafranine | - |
Desulfovibrio desulfuricans | H2 + oxidized phenosafranine | - |
? | |
H+ + reduced methyl viologen | weak activity in H2-uptake assay | Desulfovibrio desulfuricans | H2 + methyl viologen | - |
r | |
H2 + benzyl viologen | - |
Desulfovibrio desulfuricans | H+ + reduced benzyl viologen | - |
r | |
H2 + benzyl viologen | - |
Desulfovibrio desulfuricans Norway | H+ + reduced benzyl viologen | - |
r | |
H2 + ferricytochrome c3 | - |
Desulfovibrio desulfuricans | H+ + ferrocytochrome c3 | - |
r | |
H2 + ferricytochrome c3 | - |
Desulfovibrio desulfuricans Norway | H+ + ferrocytochrome c3 | - |
r | |
H2 + methylene blue | no activity in H2-evolution assay | Desulfovibrio desulfuricans | H+ + reduced methylene blue | - |
ir | |
H2 + methylene blue | no activity in H2-evolution assay | Desulfovibrio desulfuricans Norway | H+ + reduced methylene blue | - |
ir | |
additional information | cytochrome c3 is the natural electron acceptor | Desulfovibrio desulfuricans | ? | - |
? | |
additional information | cytochrome c3 is the natural electron acceptor | Desulfovibrio desulfuricans Norway | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 58000, SDS-PAGE | Desulfovibrio desulfuricans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
4 weeks, atmosphere of N2, air or H2, very little loss of activity | Desulfovibrio desulfuricans |
23 | - |
atmosphere of N2, air or H2, 40% loss of activity after 2 weeks, complete loss of activity after 4 weeks | Desulfovibrio desulfuricans |
77 | - |
stable below, 2 h | Desulfovibrio desulfuricans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
H2-evolution assay | Desulfovibrio desulfuricans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | pH 5.0: about 30% of maximal activity, pH 9.0: about 35% of maximal activity | Desulfovibrio desulfuricans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c3 | - |
Desulfovibrio desulfuricans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki for CO is 1.64 kPa | Desulfovibrio desulfuricans |