KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.341 | - |
H2 | - |
Sporomusa sphaeroides |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | cytoplasmic | Sporomusa sphaeroides | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | 15.6 mol iron per mol enzyme | Sporomusa sphaeroides | |
Ni | 0.6 mol per mol enzyme | Sporomusa sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
1 * 65000 + 1 * 37000, SDS-PAGE | Sporomusa sphaeroides |
65000 | - |
1 * 65000 + 1 * 37000, SDS-PAGE | Sporomusa sphaeroides |
122000 | - |
native PAGE | Sporomusa sphaeroides |
130000 | - |
gel filtration | Sporomusa sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sporomusa sphaeroides | - |
strain DSM 2875 | - |
Purification (Comment) | Organism |
---|---|
purified under aerobic conditions, requires reductive activation | Sporomusa sphaeroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.8 | - |
hydrogen oxidation, benzyl viologen as electron acceptor | Sporomusa sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + electron acceptor | cytochrome b as electron acceptor | Sporomusa sphaeroides | H+ + reduced electron acceptor | - |
r | |
H2 + electron acceptor | methyl viologen as electron acceptor | Sporomusa sphaeroides | H+ + reduced electron acceptor | - |
r | |
H2 + electron acceptor | benzyl viologen as electron acceptor | Sporomusa sphaeroides | H+ + reduced electron acceptor | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 65000 + 1 * 37000, SDS-PAGE | Sporomusa sphaeroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Sporomusa sphaeroides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.683 | - |
H2 | - |
Sporomusa sphaeroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Sporomusa sphaeroides |