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Literature summary for 1.12.7.2 extracted from

  • Adams, M.W.W.; Mortenson, L.E.
    The purification of hydrogenase II (uptake hydrogenase) from the anaerobic N2-fixing bacterium Clostridium pasteurianum (1984), Biochim. Biophys. Acta, 766, 51-61.
No PubMed abstract available
Search for more literature for 1.12.7.2

Metals/Ions

Metals/Ions Comment Organism Structure
Fe hydrogenase I contains 12 atoms per mol enzyme and hydrogenase II 8 atoms per mol enzyme Clostridium pasteurianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 hydrogenase II, SDS-PAGE Clostridium pasteurianum

Organism

Organism UniProt Comment Textmining
Clostridium pasteurianum
-
 hydrogenase II, uptake hydrogenase
-

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium pasteurianum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11
-
 hydrogen production, methylene blue as electron donor, hydrogenase I Clostridium pasteurianum
61
-
 hydrogen oxidation, methylene blue as electron acceptor, hydrogenase I Clostridium pasteurianum
2998
-
 hydrogen oxidation, methylene blue as electron acceptor, hydrogenase II Clostridium pasteurianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + electron acceptor methylene blue as electron acceptor Clostridium pasteurianum H+ + reduced electron acceptor
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 53000, hydrogenase II, SDS-PAGE Clostridium pasteurianum