Protein Variants | Comment | Organism |
---|---|---|
C42S | mutant enzyme with almost no activity in the reaction with H2 and oxidized benzyl viologen | Methanosarcina barkeri |
C45S | mutants with about 5% of the wild-type activity in the reaction with H2 and oxidized benzyl viologen | Methanosarcina barkeri |
C48S | mutant enzyme with almost no activity in the reaction with H2 and oxidized benzyl viologen | Methanosarcina barkeri |
C73S | mutants with about 5% of the wild-type activity in the reaction with H2 and oxidized benzyl viologen | Methanosarcina barkeri |
C76S | mutant enzyme with almost no activity in the reaction with H2 and oxidized benzyl viologen | Methanosarcina barkeri |
C79S | mutants with about 5% of the wild-type activity in the reaction with H2 and oxidized benzyl viologen | Methanosarcina barkeri |
C83S | mutant enzyme with approximately 10% of the activity of the wild-type enzyme in the reaction with H2 and oxidized benzyl viologen | Methanosarcina barkeri |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound to | Methanosarcina barkeri | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + ferredoxin + oxidized metronidazole | - |
Methanosarcina barkeri | H+ + ferredoxin + reduced metronidazole | - |
? | |
H2 + oxidized benzyl viologen | wild-type enzyme catalysed the reduction of benzylviologen at fourfold higher rates than the reduction of ferredoxin | Methanosarcina barkeri | reduced benzyl viologen + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ech hydrogenase | - |
Methanosarcina barkeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
iron-sulfur centre | the three [4Fe-4S] clusters simultaneously mediate electron and proton transfer and thus could be an essential part of the proton-translocating machinery | Methanosarcina barkeri |