Activating Compound | Comment | Organism | Structure |
---|---|---|---|
FAD | reactivation without added FAD restores only methyl viologen-dependent activity, no activity with F420 | Methanobacterium formicicum | |
KCl | 0.8 M activate F420-linked activity more than 10fold, slight inhibition of methyl viologen linked activity | Methanobacterium formicicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
coenzyme F420 | - |
Methanobacterium formicicum | |
0.08 | - |
coenzyme F0 | - |
Methanobacterium formicicum | |
0.087 | - |
FAD | - |
Methanobacterium formicicum | |
1.56 | - |
methyl viologen | - |
Methanobacterium formicicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | - |
Methanobacterium formicicum | |
Nickel | - |
Methanobacterium formicicum | |
selenium | no selenium detected | Methanobacterium formicicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
102000 | - |
smallest active species, gel filtration | Methanobacterium formicicum |
109000 | - |
gel filtration | Methanobacterium formicicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + coenzyme F420 | Methanobacterium formicicum | enzyme of methanogenesis pathway | reduced coenzyme F420 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanobacterium formicicum | - |
- |
- |
Oxidation Stability | Organism |
---|---|
purified enzyme requires reductive reactivation before assay, reactivation possible with H2 or glucose and glucose oxidase | Methanobacterium formicicum |
Purification (Comment) | Organism |
---|---|
- |
Methanobacterium formicicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
97.6 | - |
purified enzyme with F420 | Methanobacterium formicicum |
98.6 | - |
purified enzyme with F0 | Methanobacterium formicicum |
172 | - |
purified enzyme with methyl viologen | Methanobacterium formicicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + coenzyme F0 | - |
Methanobacterium formicicum | reduced coenzyme F0 | - |
? | |
H2 + coenzyme F420 | - |
Methanobacterium formicicum | reduced coenzyme F420 | - |
r | |
H2 + coenzyme F420 | enzyme of methanogenesis pathway | Methanobacterium formicicum | reduced coenzyme F420 | - |
r | |
H2 + FAD | - |
Methanobacterium formicicum | H+ + FADH2 | - |
r | |
H2 + methyl viologen | - |
Methanobacterium formicicum | ? | - |
? | |
additional information | reduces different flavins | Methanobacterium formicicum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | alpha,beta,gamma 43600 + 36700 + 28000, SDS-PAGE, exact subunit composition not known, ratio 1:1:1 postulated | Methanobacterium formicicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Methanobacterium formicicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Methanobacterium formicicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | - |
Methanobacterium formicicum |
5.5 | 8 | reverse reaction | Methanobacterium formicicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol per mol 109000 Da species | Methanobacterium formicicum | |
FAD | FAD depleted enzyme reduces methyl viologen but not F420 | Methanobacterium formicicum | |
additional information | no activity with FMN after FAD depletion | Methanobacterium formicicum |