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Literature summary for 1.12.98.4 extracted from
- Characterization of hydrogenase II from the hyperthermophilic archaeon Pyrococcus furiosus and assessment of its role in sulfur reduction (2000), J. Bacteriol., 182, 1864-1871.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | contains 21 atoms iron per mol enzyme | Pyrococcus furiosus |  |
| Ni2+ | contains 0.9 atoms nickel per mol enzyme | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
| 30000 | - |
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
| 39000 | - |
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
| 52000 | - |
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
| 320000 | - |
gel filtration | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | E7FHC4 and E7FHN9 and E7FHW8 and E7FHF8 | subunits alpha, beta, gamma and delta | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE column chromatography, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + benzyl viologen | highest activity | Pyrococcus furiosus | H2S + reduced benzyl viologen | - |
? | |
| H2 + methyl viologen | - |
Pyrococcus furiosus | H2S + reduced methyl viologen | - |
? | |
| H2 + polysulfide(n) | - |
Pyrococcus furiosus | H2S + polysulfide(n-1) | - |
r | |
| additional information | ferredoxin from Pyrococcus furiosus is not an efficient electron carrier | Pyrococcus furiosus | ? | - |
? | |
| NADPH + H+ + polysulfide(n) | - |
Pyrococcus furiosus | NADP+ + polysulfide(n-1) | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| H-II | - |
Pyrococcus furiosus |
| hydrogenase II | - |
Pyrococcus furiosus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains 0.83 FAD per mol enzyme | Pyrococcus furiosus | |
| NADH | NADPH and NADH are equally efficient as electron donors for H2 production | Pyrococcus furiosus | |
| NADPH | NADPH and NADH are equally efficient as electron donors for H2 production | Pyrococcus furiosus | |
| [2Fe-2S]-center | contains 1 [2Fe-2S] center | Pyrococcus furiosus | |
| [4Fe-4S]-center | contains 2 [4Fe-4S]-centers | Pyrococcus furiosus | |
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