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Literature summary for 1.12.99.6 extracted from

  • Santiago, B.; Meyer, O.
    Purification and molecular characterization of the H2 uptake membrane-bound NiFe-hydrogenase from the carboxidotrophic bacterium Oligotropha carboxidovorans (1997), J. Bacteriol., 179, 6053-6060.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.12.99.6

Cloned(Commentary)

Cloned (Comment) Organism
-
 Afipia carboxidovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 7.3 mol per enzyme Afipia carboxidovorans
Ni 0.725 mol per enzyme Afipia carboxidovorans
Sulfide 7.2 mol labile sulfide per enzyme Afipia carboxidovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 1 * 69000 + 1 * 30000, SDS-PAGE Afipia carboxidovorans
69000
-
 1 * 69000 + 1 * 30000, SDS-PAGE Afipia carboxidovorans
101700
-
 calculated from amino acid sequence Afipia carboxidovorans
102000
-
 sucrose density gradient centrifugation Afipia carboxidovorans
105000
-
 gel filtration Afipia carboxidovorans
128000
-
 native PAGE Afipia carboxidovorans

Organism

Organism UniProt Comment Textmining
Afipia carboxidovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Afipia carboxidovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
173 178
-
 Afipia carboxidovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + acceptor enzyme acts on artificial electron-accepting dyes, but is ineffective with pyridine nucleotides or other soluble physiological electron acceptors Afipia carboxidovorans H+ + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 69000 + 1 * 30000, SDS-PAGE Afipia carboxidovorans