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Literature summary for 1.12.99.6 extracted from
- Characterization of the menaquinone reduction site in the diheme cytochrome b membrane anchor of Wolinella succinogenes NiFe-hydrogenase (2004), J. Biol. Chem., 279, 274-281.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of Strep-tagged enzyme | Wolinella succinogenes |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A204F | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
| D100N | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
| D88N | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
| E94Q | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
| G125L | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
| H186A | no acitivity with 2,3-dimethyl1,4-naphthoquinone | Wolinella succinogenes |
| H200A | no acitivity with 2,3-dimethyl1,4-naphthoquinone | Wolinella succinogenes |
| H25A | no acitivity with 2,3-dimethyl1,4-naphthoquinone | Wolinella succinogenes |
| H67A | no acitivity with 2,3-dimethyl1,4-naphthoquinone | Wolinella succinogenes |
| M203I | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
| N128D | 5% of wild-type activity with 2,3-dimethyl1,4-naphthoquinone | Wolinella succinogenes |
| P129A | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
| Q131L | 2% of wild-type activity with 2,3-dimethyl1,4-naphthoquinone | Wolinella succinogenes |
| Y114F | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
| Y127A | 9% of wild-type activity with 2,3-dimethyl1,4-naphthoquinone | Wolinella succinogenes |
| Y127F | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
| Y127H | no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type | Wolinella succinogenes |
| Y202F | almost no activity with 2,3-dimethyl1,4-naphthoquinone | Wolinella succinogenes |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-bound, enzyme is oriented to the periplasmic space | Wolinella succinogenes | 16020 | - |
| periplasm | - |
Wolinella succinogenes | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | FeNi-hydrogenase | Wolinella succinogenes |  |
| Ni | FeNi-hydrogenase | Wolinella succinogenes | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Wolinella succinogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Wolinella succinogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + 2,3-dimethyl-1,4-naphthoquinone | - |
Wolinella succinogenes | 2,3-dimethyl-naphthoquinol | - |
? | |
| H2 + oxidized benzyl viologen | - |
Wolinella succinogenes | reduced benzyl viologen + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | subunits HydA, HydB and HydC | Wolinella succinogenes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme b | involved in electron transport | Wolinella succinogenes | |
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