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Literature summary for 1.12.99.6 extracted from
- Escherichia coli hydrogenase 3 is a reversible enzyme possessing hydrogen uptake and synthesis activities (2007), Appl. Microbiol. Biotechnol., 76, 1035-1042.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the uptake activity in mutant HD705/pBS(Kan), which is defective in hydrogenase 3, is 4.4fold lower than that in wild-type enzyme, the hydrogen uptake activity in the hydrogenase 1 and 2 double mutant (hyaB hybC) is reduced 2.7fold by addition of the hycE mutation (hyaB hybC hycE) | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CN- | contains two CN- molecules at the active site | Escherichia coli |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
hydrogenase 3, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
strains BW25113 and MC4100 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.008 | - |
hydrogen uptake specific activity of the hydrogenase 3 lacking mutant enzyme HD705/pBS(Kan), using methyl viologen as a substrate | Escherichia coli |
| 0.035 | - |
hydrogen uptake specific activity of the wild type enzyme, using methyl viologen as a substrate | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + acceptor | - |
Escherichia coli | acceptor-H2 | - |
? | |
| H2 + oxidized methyl viologen | - |
Escherichia coli | H+ + reduced methyl viologen | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HycE | hydrogenase 3 | Escherichia coli |
| hydrogen uptake hydrogenase | - |
Escherichia coli |
| hydrogenase 1 | hydrogen uptake activity only | Escherichia coli |
| hydrogenase 2 | hydrogen uptake activity only | Escherichia coli |
| hydrogenase 3 | hydrogen uptake and synthesis activity | Escherichia coli |
| [NiFe]-hydrogenase | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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