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Literature summary for 1.12.99.6 extracted from
- Protein engineering of hydrogenase 3 to enhance hydrogen production (2008), Appl. Microbiol. Biotechnol., 79, 77-86.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| variants expressed from epPCR and DNA shuffling plasmid pBS(Kan)HycE in Escherichia coli BW25113 hyaB hybC hycE DELTAkan | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D202V/K492 | variant epHycE70, has 11fold higher hydrogen production and 7fold higher hydrogen yield from formate compared to wild-type | Escherichia coli |
| D210N/I271F/K545R | variant epHycE23-2, has 8fold higher hydrogen production and 4fold higher hydrogen yield from formate compared to wild-type | Escherichia coli |
| F297L/L327Q/E382K/L415M/A504T/D542N | variant epHycE17, has 7fold higher hydrogen production and 4fold higher hydrogen yield from formate compared to wild-type | Escherichia coli |
| I333F/K554d | variant epHycE39, has 7fold higher hydrogen production and 3fold higher hydrogen yield from formate compared to wild-type | Escherichia coli |
| additional information | saturation mutagenesis at T366 of HycE leads to increased hydrogen production via a truncation at this position, 204 amino acids at the carboxy terminus may be deleted to increase hydrogen production by 30fold | Escherichia coli |
| Q32R/V112L/G245C/F409L | variant epHycE21, has 15fold higher hydrogen production and 6fold higher hydrogen yield from formate compared to wild-type | Escherichia coli |
| S2P/E4G/M314V/T366S/V394D/S397C | variant epHycE67, has 13fold higher hydrogen production and 5fold higher hydrogen yield from formate compared to wild-type | Escherichia coli |
| S2T/Y50F/I171T/A291V/T366S/V433L/M444I/L523Q | shows a 17fold higher hydrogen-producing activity and 8fold higher hydrogen yield from formate than wild type HycE | Escherichia coli |
| S2T/Y50F/I171T/A291V/T366S/V433L/M444I/L523Q | variant epHycE95, has 17fold higher hydrogen-producing activity and 8fold higher hydrogen yield from formate compared to wild-type | Escherichia coli |
| T366 | variant satHycE12T366, has 30fold higher hydrogen production compared to wild-type | Escherichia coli |
| T366 | variant satHycE19T366, has 27fold higher hydrogen production compared to wild-type | Escherichia coli |
| Y464 | variant shufHycE1-9, has 23fold higher hydrogen production and 9fold higher hydrogen yield from formate compared to wild-type | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | contains a Fe2+-binding site | Escherichia coli |  |
| Ni2+ | contains a Ni2+-binding site | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli | - |
K-12 BW25113 | - |
| Escherichia coli K-12 BW25113 | - |
- |
- |
| Escherichia coli K-12 BW25113 | - |
K-12 BW25113 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formate | - |
Escherichia coli | H2 + CO2 | - |
r | |
| formate | - |
Escherichia coli K-12 BW25113 | H2 + CO2 | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HycE | - |
Escherichia coli |
| hydrogenase 3 | - |
Escherichia coli |
| hydrogenase 3 | hydrogenase 3 is the large subunit of a [Ni-Fe]-type hydrogenase | Escherichia coli |
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Release 2023.1 (January 2023)
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