Activating Compound | Comment | Organism | Structure |
---|---|---|---|
HydE | can coordinate variable amounts of Fe and sulfide | Thermotoga maritima | |
HydE | is required to synthesize and assemble the active site utilizing Escherichia coli metabolites | Clostridium acetobutylicum | |
HydEF | maturase, role in biosynthesis of the active site. HydEF and hydG, coexpressed with the structural genes hydA1 and hydA2 in Escherichia coli lead to active [FeFe]-hydrogenase | Chlamydomonas reinhardtii | |
HydF | - |
Thermotoga maritima | |
HydF | is required to synthesize and assemble the active site utilizing Escherichia coli metabolites | Clostridium acetobutylicum | |
HydG | is required to synthesize and assemble the active site utilizing Escherichia coli metabolites | Clostridium acetobutylicum | |
HydG | maturase, role in biosynthesis of the active site | Desulfovibrio vulgaris | |
HydG | maturase, role in biosynthesis of the active site. HydEF and hydG, coexpressed with the structural genes hydA1 and hydA2 in Escherichia coli lead to active [FeFe]-hydrogenase | Chlamydomonas reinhardtii |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Desulfovibrio vulgaris |
hydA1 and hydA2 expressed in Escherichia coli | Chlamydomonas reinhardtii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | lacks the ferredoxin-like clusters, but contains the H-cluster [Fe4S4] component | Chlamydomonas reinhardtii | |
Fe | two ferredoxin-like [Fe4S4] clusters | Desulfovibrio vulgaris | |
Fe | [FeS] cluster | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Clostridium acetobutylicum | - |
- |
- |
Desulfovibrio vulgaris | - |
- |
- |
Thermotoga maritima | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
HydA1 | - |
Chlamydomonas reinhardtii |
HydA2 | - |
Chlamydomonas reinhardtii |
[FeFe]-hydrogenase | - |
Chlamydomonas reinhardtii |
[FeFe]-hydrogenase | - |
Clostridium acetobutylicum |
[FeFe]-hydrogenase | - |
Desulfovibrio vulgaris |
[FeFe]-hydrogenase | - |
Thermotoga maritima |
General Information | Comment | Organism |
---|---|---|
malfunction | heterologous expression of the two structural genes of [FeFe]-hydrogenase in Escherichia coli yields an inactive enzyme, only the two ferredoxin-like [Fe4S4] clusters are unambiguously assembled | Desulfovibrio vulgaris |