Literature summary for 1.12.99.6 extracted from

Kuchenreuther, J.M.; Stapleton, J.A.; Swartz, J.R.
Tyrosine, cysteine, and S-adenosyl methionine stimulate in vitro [FeFe] hydrogenase activation (2009), PLoS ONE, 4, e7565.

Search for more literature for 1.12.99.6

Activating Compound

Activating Compound	Comment	Organism	Structure
3,4-dihydroxy-L-phenylalanine	substitutes for tyrosine to stimulate in vitro HydA1 activation, improves HydA1 maturation 4fold	Chlamydomonas reinhardtii
additional information	guanosine triphosphate does not improve hydrogenase maturation	Chlamydomonas reinhardtii
additional information	tyrosine enhances in vitro maturation of HydA1 hydrogenase. Tyrosine has the most significant effect on overall HydA1 activities. Cysteine enhances in vitro maturation of HydA1 hydrogenase. Cysteine has the most significant effect on early HydA1 activities	Chlamydomonas reinhardtii
S-adenosyl methionine	exogenous S-adenosyl methionine stimulates in vitro hydrogenase activation, but does not individually enhance hydrogenase activation. 2 mM increases hydrogenase activity 4fold	Chlamydomonas reinhardtii
Sulfide	1 mM increases hydrogenase activity 4fold, is not sufficient to increase hydrogenase activities without S-adenosyl methionine and the standard 20 L-amino acids	Chlamydomonas reinhardtii

Cloned(Commentary)

Cloned (Comment)	Organism
heterologously expressed in Escherichia coli BL21(DE3) from vector pY71 N-His6-shydA1*	Chlamydomonas reinhardtii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	1 mM increases hydrogenase activity 4fold, is not sufficient to increase hydrogenase activities without S-adenosyl methionine and the standard 20 L-amino acids	Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48400	-	x * 48400, SDS-PAGE, N-His6-HydA1	Chlamydomonas reinhardtii

Organism

Organism	UniProt	Comment	Textmining
Chlamydomonas reinhardtii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by immobilized metal-affinity chromatography	Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2 + oxidized methyl viologen	-	Chlamydomonas reinhardtii	H+ + reduced methyl viologen	-	?

Subunits

Subunits	Comment	Organism
?	x * 48400, SDS-PAGE, N-His6-HydA1	Chlamydomonas reinhardtii

Synonyms

Synonyms	Comment	Organism
HydA1	-	Chlamydomonas reinhardtii
[FeFe]-hydrogenase	-	Chlamydomonas reinhardtii

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Release 2023.1 (January 2023)