Any feedback?
Literature summary for 1.12.99.6 extracted from
- Pressure stabilization of proteins from extreme thermophiles (1994), Appl. Environ. Microbiol., 60, 932-939.
General Stability
| General Stability | Organism |
|---|---|
| pressure of 50.7 MPa destabilizes the enzyme | Methanothermococcus thermolithotrophicus |
| pressure of 50.7 MPa destabilizes the enzyme | Methanococcus maripaludis |
| pressure of 50.7 MPa has a substantial stabilizing effect on the enzyme | Methanocaldococcus jannaschii |
| pressure of 50.7 MPa has a substantial stabilizing effect on the enzyme | Methanotorris igneus |
| the half-life for inactivation of the hydrogenase at pressure of 1.0 MPa is 28 min | Methanococcus maripaludis |
| the half-life for inactivation of the hydrogenase at pressure of 1.0 MPa is 30 min | Methanocaldococcus jannaschii |
| the half-life for inactivation of the hydrogenase at pressure of 1.0 MPa is 49 min | Methanothermococcus thermolithotrophicus |
| the half-life for inactivation of the hydrogenase at pressure of 1.0 MPa is 96 min | Methanotorris igneus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
- |
- |
| Methanococcus maripaludis | - |
- |
- |
| Methanothermococcus thermolithotrophicus | - |
- |
- |
| Methanotorris igneus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Methanothermococcus thermolithotrophicus |
| partial | Methanocaldococcus jannaschii |
| partial | Methanococcus maripaludis |
| partial | Methanotorris igneus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + oxidized methyl viologen | - |
Methanothermococcus thermolithotrophicus | H+ + reduced methyl viologen | - |
? |  |
| H2 + oxidized methyl viologen | - |
Methanocaldococcus jannaschii | H+ + reduced methyl viologen | - |
? | |
| H2 + oxidized methyl viologen | - |
Methanococcus maripaludis | H+ + reduced methyl viologen | - |
? | |
| H2 + oxidized methyl viologen | - |
Methanotorris igneus | H+ + reduced methyl viologen | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
an increase in pressure from 1.0 to 50.7 MPa increases the half-life of the hydrogenase at 90°C by about 400% | Methanocaldococcus jannaschii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Methanothermococcus thermolithotrophicus |
| 7.5 | - |
assay at | Methanocaldococcus jannaschii |
| 7.5 | - |
assay at | Methanococcus maripaludis |
| 7.5 | - |
assay at | Methanotorris igneus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
