Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 0.1 mM, enzyme from aniline-grown cells loses 88% of its activity, enzyme from L-malate-grown cells loses 67% of its activity | Rhodococcus sp. | |
HgCl2 | enzyme from aniline-grown cells completely loses activity, enzyme from L-malate-grown cells loses 98% of its activity | Rhodococcus sp. | |
PCMB | 1.0 mM, enzyme from aniline-grown cells loses 20% of its activity, enzyme from L-malate-grown cells loses 10% of its activity | Rhodococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
1 * 32000, enzyme from aniline-grown cells and from L-malate-grown cells, SDS-PAGE | Rhodococcus sp. |
33000 | - |
enzyme from aniline-grown cells and from L-malate-grown cells, gel filtration | Rhodococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rhodococcus erythropolis | inducible on aniline, not on L-malate | ? | - |
? | |
additional information | Frateuria sp. | inducible on aniline, not on L-malate | ? | - |
? | |
additional information | Arthrobacter sp. | inducible on benzamide, not on L-malate | ? | - |
? | |
additional information | Rhodococcus sp. | synthesized constitutively. When the bacterium is incubated on a medium with D-glucose, L-malate, isoleucine, leucine, etc., it synthesizes more catechol 1,2-dioxygenase than that in cells grown on aniline | ? | - |
? | |
additional information | Frateuria sp. ANA-18 | inducible on aniline, not on L-malate | ? | - |
? | |
additional information | Arthrobacter sp. BA-5-17 | inducible on benzamide, not on L-malate | ? | - |
? | |
additional information | Rhodococcus erythropolis AN-13 | inducible on aniline, not on L-malate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
BA-5-17 | - |
Arthrobacter sp. BA-5-17 | - |
BA-5-17 | - |
Frateuria sp. | - |
ANA-18 | - |
Frateuria sp. ANA-18 | - |
ANA-18 | - |
Rhodococcus erythropolis | - |
AN-13 | - |
Rhodococcus erythropolis AN-13 | - |
AN-13 | - |
Rhodococcus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from aniline-grown cells and from L-malate-grown cells | Rhodococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34 | - |
enzyme purified from L-malate grown cells | Rhodococcus sp. |
35 | - |
enzyme purified from aniline-grown cells | Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | enzyme from aniline-grown cells: 74% of the activity with catechol, enzyme from L-malate-grown cells: 71% of the activity with catechol | Rhodococcus sp. | 2-methyl-cis,cis-muconate | - |
? | |
4-methylcatechol + O2 | enzyme from aniline-grown cells: 86% of the activity with catechol, enzyme from L-malate-grown cells: 87% of the activity with catechol | Rhodococcus sp. | 3-methyl-cis,cis-muconate | - |
? | |
catechol + O2 | - |
Arthrobacter sp. | cis,cis-muconate | - |
? | |
catechol + O2 | - |
Rhodococcus sp. | cis,cis-muconate | - |
? | |
catechol + O2 | - |
Rhodococcus erythropolis | cis,cis-muconate | - |
? | |
catechol + O2 | - |
Frateuria sp. | cis,cis-muconate | - |
? | |
catechol + O2 | - |
Frateuria sp. ANA-18 | cis,cis-muconate | - |
? | |
catechol + O2 | - |
Arthrobacter sp. BA-5-17 | cis,cis-muconate | - |
? | |
catechol + O2 | - |
Rhodococcus erythropolis AN-13 | cis,cis-muconate | - |
? | |
additional information | inducible on aniline, not on L-malate | Rhodococcus erythropolis | ? | - |
? | |
additional information | inducible on aniline, not on L-malate | Frateuria sp. | ? | - |
? | |
additional information | inducible on benzamide, not on L-malate | Arthrobacter sp. | ? | - |
? | |
additional information | synthesized constitutively. When the bacterium is incubated on a medium with D-glucose, L-malate, isoleucine, leucine, etc., it synthesizes more catechol 1,2-dioxygenase than that in cells grown on aniline | Rhodococcus sp. | ? | - |
? | |
additional information | inducible on aniline, not on L-malate | Frateuria sp. ANA-18 | ? | - |
? | |
additional information | inducible on benzamide, not on L-malate | Arthrobacter sp. BA-5-17 | ? | - |
? | |
additional information | inducible on aniline, not on L-malate | Rhodococcus erythropolis AN-13 | ? | - |
? | |
pyrogallol + O2 | - |
Rhodococcus sp. | alpha-hydroxymuconic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 32000, enzyme from aniline-grown cells and from L-malate-grown cells, SDS-PAGE | Rhodococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
enzyme maintains more than 80% of its activity | Rhodococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
enzyme from L-malate grown cells | Rhodococcus sp. |
8.5 | - |
enzyme from aniline-grown cells | Rhodococcus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 11.6 | enzyme maintains more than 90% of its activity | Rhodococcus sp. |