Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | non-heme iron of the enzyme | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
isozyme 3, gel filtration | Pseudomonas putida |
64000 | - |
isozyme 2, gel filtration | Pseudomonas putida |
66900 | - |
isozyme 1, gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | Pseudomonas putida | - |
cis,cis-muconate | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
hexane | isozyme 1 is able to function in a non-aqueous environment catalyzing the oxidation of catechol in n-hexane, lyophilized isozyme in n-hexane, overview | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
strain DSM 437, isozymes 1-3 | - |
Purification (Comment) | Organism |
---|---|
native isozymes 1-3 by ammonium sulfate fractionation, and affinity and anion exchange chromatography | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.87 | - |
crude enzyme | Pseudomonas putida |
12 | - |
purified isozyme 3 | Pseudomonas putida |
17.9 | - |
purified isozyme 2 | Pseudomonas putida |
22.4 | - |
purified isozyme 1 | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | - |
Pseudomonas putida | cis,cis-muconate | - |
? | |
catechol + O2 | isozyme1 is able to function as lyophilized enzyme in a non-aqueous environment catalyzing the oxidation of catechol in n-hexane, overview | Pseudomonas putida | cis,cis-muconate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pyrocatechase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, in aqueous medium | Pseudomonas putida |
35 | - |
assay at, in n-hexane | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at, in aqueous medium | Pseudomonas putida |
8.5 | - |
assay at, in n-hexane | Pseudomonas putida |