Cloned (Comment) | Organism |
---|---|
Escherichia coli BL21 cells carrying the plasmid PT7-7/L1 VTSLO-11 | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
11-thialinoleic acid | is a competitive inhibitor with respect to linoleic acid and a noncompetitive inhibitor with respect to arachidonic acid | Glycine max | |
14-thialinoleic acid | is a competitive inhibitor with respect to linoleic acid | Glycine max | |
additional information | presence of inhibitor does not change the regioselectivity of lipoxygenase-1 | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
linoleic acid | - |
Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by sonication, centrifugation and gel filtration | Glycine max |
Storage Stability | Organism |
---|---|
-80°C, 100 mM borate buffer, pH 10, 30% glycerol | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleic acid + O2 | - |
Glycine max | (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipoxygenase-1 | - |
Glycine max |
LO-1 | - |
Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Glycine max |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
11-thialinoleic acid | - |
Glycine max | |
0.035 | - |
14-thialinoleic acid | - |
Glycine max |