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Literature summary for 1.13.11.12 extracted from
- Characterisation of lipoxygenase isoforms from olive callus cultures (2008), Phytochemistry, 69, 2532-2538.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.043 | - |
linoleic acid | 55fold purified enzyme, at pH 8.8, isoform 2 | Olea europaea |  |
| 0.08 | - |
linoleic acid | 48fold purified enzyme, at pH 8.8, isoform 1 | Olea europaea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 95000 | - |
Western blotting and gel filtration | Olea europaea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Olea europaea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by ammonium sulphate precipitation and ion-exchange chromatography, 55fold with a yield of 5.6% | Olea europaea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| callus | - |
Olea europaea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.2 | - |
crude extract, with linoleic acid as substrate | Olea europaea |
| 12 | - |
crude extract, with alpha-linolenic acid as substrate | Olea europaea |
| 474 | - |
55fold purified enzyme, with alpha-linolenic acid as substrate | Olea europaea |
| 564 | - |
55fold purified enzyme, with linoleic acid as substrate | Olea europaea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-linolenic acid + O2 | - |
Olea europaea | ? | - |
? | |
| linoleic acid + O2 | - |
Olea europaea | (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LOX | - |
Olea europaea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
for alpha-linolenic acid | Olea europaea |
| 8.8 | - |
for linoleic acid | Olea europaea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8.4 | at pH 7.8, ca. 50% of maximal activity, at pH 8.4, ca. 30% of maximal activity, substrate alpha-linoleic acid | Olea europaea |
| 8.5 | 8.9 | at pH 8.5, ca. 30% of maximal activity, at pH 8.9, ca. 80% of maximal activity, substrate linolenic acid | Olea europaea |
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Release 2023.1 (January 2023)
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