Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.12 extracted from

  • Wang, R.; Shen, W.; Liu, L.; Jiang, L.; Liu, Y.; Su, N.; Wan, J.
    A novel lipoxygenase gene from developing rice seeds confers dual position specificity and responds to wounding and insect attack (2008), Plant Mol. Biol., 66, 401-414.
    View publication on PubMed

Application

Application Comment Organism
additional information the manipulating of the expression of the LOX gene may provide a basis for advancing both the fundamental and applied biology of rice Oryza sativa

Cloned(Commentary)

Cloned (Comment) Organism
LOX1 ORF cloned into vector pET30a(+) for overexpression in Escherichia coli. LOX1-green GFP fusion construct, under the control of the cauliflower mosaic virus 35S promoter, expressed in Arabidopsis plants. Transgenic rice lines carrying either sense or antisense constructs under the control of a cauliflower mosaic virus 35S promoter Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Oryza sativa 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
1 * 105000, SDS-PAGE, His-tagged enzyme Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa Q27PX2
-
-

Purification (Commentary)

Purification (Comment) Organism
soluble LOX purified with His bind kits Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
seed low abundance in immature seeds Oryza sativa
-
seedling low abundance in newly germinated seedlings Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleate + O2
-
Oryza sativa (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 105000, SDS-PAGE, His-tagged enzyme Oryza sativa

Synonyms

Synonyms Comment Organism
linoleate:oxygen oxidoreductase
-
Oryza sativa
LOX1
-
Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
Oryza sativa

Expression

Organism Comment Expression
Oryza sativa in all antisense transgenic rice lines and more than half of the sense lines the expression levels of LOX1, the levels of enzyme activity, and the levels of the endogenous LOX1 products (jasmonic acid, (Z)-3-hexenal and colneleic acid) at 6, 48, and 48 h after brown planthopper feeding respectively, are below the levels found in non-transgenic control plants down
Oryza sativa LOX1 transcripts accumulate rapidly and transiently in response to wounding or brown planthopper attack, reaching a peak 3 h after wounding and 6 h after insect feeding. Transgenic rice lines show altered LOX1 activity up

General Information

General Information Comment Organism
physiological function LOX1 is a non-conventional LOX with 13- or dual position-specific LOX activity. LOX1 is involved in the synthesis of jasmonic acid, colneleic acid, and (Z)-3-hexenal. The LOX1 product is involved in tolerance of the rice plant to wounding and brown planthopper Nilaparvata lugens attack Oryza sativa