Application | Comment | Organism |
---|---|---|
additional information | the manipulating of the expression of the LOX gene may provide a basis for advancing both the fundamental and applied biology of rice | Oryza sativa |
Cloned (Comment) | Organism |
---|---|
LOX1 ORF cloned into vector pET30a(+) for overexpression in Escherichia coli. LOX1-green GFP fusion construct, under the control of the cauliflower mosaic virus 35S promoter, expressed in Arabidopsis plants. Transgenic rice lines carrying either sense or antisense constructs under the control of a cauliflower mosaic virus 35S promoter | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Oryza sativa | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105000 | - |
1 * 105000, SDS-PAGE, His-tagged enzyme | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q27PX2 | - |
- |
Purification (Comment) | Organism |
---|---|
soluble LOX purified with His bind kits | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | low abundance in immature seeds | Oryza sativa | - |
seedling | low abundance in newly germinated seedlings | Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleate + O2 | - |
Oryza sativa | (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 105000, SDS-PAGE, His-tagged enzyme | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
linoleate:oxygen oxidoreductase | - |
Oryza sativa |
LOX1 | - |
Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
- |
Oryza sativa |
Organism | Comment | Expression |
---|---|---|
Oryza sativa | in all antisense transgenic rice lines and more than half of the sense lines the expression levels of LOX1, the levels of enzyme activity, and the levels of the endogenous LOX1 products (jasmonic acid, (Z)-3-hexenal and colneleic acid) at 6, 48, and 48 h after brown planthopper feeding respectively, are below the levels found in non-transgenic control plants | down |
Oryza sativa | LOX1 transcripts accumulate rapidly and transiently in response to wounding or brown planthopper attack, reaching a peak 3 h after wounding and 6 h after insect feeding. Transgenic rice lines show altered LOX1 activity | up |
General Information | Comment | Organism |
---|---|---|
physiological function | LOX1 is a non-conventional LOX with 13- or dual position-specific LOX activity. LOX1 is involved in the synthesis of jasmonic acid, colneleic acid, and (Z)-3-hexenal. The LOX1 product is involved in tolerance of the rice plant to wounding and brown planthopper Nilaparvata lugens attack | Oryza sativa |